慢性鼻窦炎伴嗅觉障碍患者嗅黏膜中PCNA与LNGFr表达的改变  

Expression of PCNA and LNGFr in olfactory epithelium of patients suffering from dysosmia caused by chronic sinusitis

作　　者：李力[1] 张燕[2] 蒲建章[3] 李桂芳[4] 叶鸣[5] 

机构地区：[1]北京市顺义区医院耳鼻咽喉科北京,101300 [2]河北医科大学第二医院耳鼻咽喉科 [3]北京市顺义区医院神经外科 [4]河北邢台市眼科医院耳鼻咽喉科 [5]中国人民解放军军械工程学院门诊部

出　　处：《临床耳鼻咽喉头颈外科杂志》2012年第11期502-506,共5页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

摘　　要：目的:研究增殖细胞核抗原(PCNA)、低亲和力神经生长因子受体(LNGFr)在慢性鼻窦炎伴嗅觉障碍患者嗅觉神经元(ORNs)的表达以及LNGFr的调控作用。方法:采用CCCRC方法对46例行功能性鼻内镜手术治疗的患者进行嗅觉评分并分为3组:A组为慢性鼻窦炎伴嗅觉障碍25例;B组为慢性鼻窦炎不伴嗅觉障碍10例;C组为单纯鼻中隔偏曲行鼻中隔矫正术11例。采用免疫组织化学方法检测PCNA和LNGFr在3组患者嗅黏膜中的表达。结果:PCNA和LNGFr在A组嗅黏膜基底细胞中的表达显著高于B组(P<0.01)和C组(P<0.01);A组中,PCNA在基底细胞中表达的阳性细胞数与嗅觉评分呈负相关(r=-0.7441,P<0.01);LNG-Fr着色强度的积分光度值与嗅觉评分呈负相关(r=-0.440 7,P<0.05);LNGFr着色强度的积分光度值与PCNA的阳性细胞数呈正相关(r=0.5317,P<0.01)。结论:慢性鼻窦炎伴嗅觉障碍患者嗅黏膜中神经干细胞即基底细胞大量增殖,其增殖能力的增强与LNGFr的表达上调有关。Objective：To study the expression of PCNA and LNGFr in olfactory epithelium of patients suffering from dysosmia caused by chronic sinusitis,and the function of LNGFr.Method：Forty-six patients undergoing FESS were chosen.Before operation,their olfactory functions were examined with CCCRC.According to their CCCRC scores,they were divided into three groups.Group A： Patients with chronic sinusitis and dysosmia 25 cases;Group B： Patients with chronic sinusitis and a normal olfactory function 10 cases;Group C： Patients with deviation of nasal septum and a normal olfactory function 11 cases.The expressions of PCNA and LNGFr were measured in olfactory mucosas of the three groups by immun-ohistochemistry.Result：In basal cells,the expression of PCNA and LNGFr in group A was higher than that in group B（P0.01）.and in group C（P0.01）.There was negative correlation between positive cells of PCNA and CCCRC score in basal cells of group A（r=-0.744 1,P0.01）;There was negative correlation between integral optical density of LNGFr and CCCRC score in basal cells of group A（r=-0.440 7,P0.05）.There was positive correlation between positive cells of PCNA and integral optical density of LNGFr in basal cells of group A（r=0.531 7,P0.01）.Conclusion：Basal cells proliferated dramatically in patients suffering from dysosmia caused by chronic sinusitis.The proliferating capacity of basal cells was related to up-regulation of LNGFr expression.

关 键 词：慢性鼻窦炎 嗅觉障碍 嗅觉神经元 再生 

分 类 号：R765.4[医药卫生—耳鼻咽喉科]