单细胞凝胶电泳技术评价毛鸡骨草的遗传毒性  

Evaluation of Genotoxicity of Abrus mollis Hance by Singe Cell Gel Electrophoresis

作　　者：陈晓白[1] 王晓平[1] 岑並丽[1] 廖邦力[1] 

机构地区：[1]玉林师范学院生命科学与技术学院,广西玉林537000

出　　处：《安徽农业科学》2012年第14期8066-8067,8070,共3页Journal of Anhui Agricultural Sciences

基　　金：广西教育厅科研资助项目(200710MS052);广西玉林市科技局项目(0881038)

摘　　要：[目的]应用单细胞凝胶电泳技术评价毛鸡骨草的遗传毒性。[方法]试验分为5组:阳性对照组(环磷酰胺组)、阴性对照组(生理盐水组)、毛鸡骨草高(30 g/kg)、中(20 g/kg)、低(10 g/kg)剂量组,利用单细胞凝胶电泳技术分析小鼠肺、肾、肝、睾丸细胞的尾部DNA率(Tail DNA%)和尾矩(Tail Moment),以观察毛鸡骨草对小鼠DNA的影响。[结果]毛鸡骨草各剂量组对小鼠肺、肾、肝、睾丸细胞的Tail DNA%和Tail Moment影响明显低于阳性对照组,差异极显著(P<0.01);毛鸡骨草高剂量组肝细胞Tail DNA%和Tail Moment与阴性对照组相比明显降低,差异极显著(P<0.01);其他各剂量组小鼠细胞Tail DNA%和Tail Moment与阴性对照组比,无明显差异(P>0.05)。[结论]毛鸡骨草在试验剂量范围内对小鼠肺、肾、肝、睾丸细胞DNA无损伤。[ Objective ] To evaluate the genotoxicity of Abrus mollis Hance by single cell gel electrophoresis. [ Method ] Five groups were set up in the experiment, including positive control group （cyclophosphamide）, negative control group （normal saline）, high-dose group （30 g/kg）, moderate-dose group （20 g/kg） and low-dose group （1 0 g/kg）. The impact of Abrus mollis Hance on the Tail DNA%, Tail Moment, and DNA damage of liver, kidney, lung, testicular ceils was detected by single cell gel electrophoresis. [ Result ] Compared with positive control group, Tail DNA% and Tail Moment of liver, kidney, lung and testicular cells in Abrus molli~ Hance group were significantly lower（P 〈0.01 ）. Com- pared with the negative control group, Tail DNA% and Tail Moment of liver cells in the Abrus mollis Hance group with high dose were significant- ly lower（ P 〈 0. O1 ）, Tail Moment and Tail DNA% of other cells in other dose groups were not significantly different （P 〉 O. 05 ）. [ Conclusion ] Abrus mollis Hance had no damage to the DNA in mouse lung, liver, kidney and testicular cells within the doses in experiment.

关 键 词：毛鸡骨草 单细胞凝胶电泳 遗传毒性 

分 类 号：S567[农业科学—中草药栽培]