致病疫霉拮抗芽孢杆菌的筛选及脂肽类物质分离的研究  

Screening of Antagonistic Bacillus spp.Strains against Phytophthora infestans(Mont.) de Bary and Isolation of Lipopeptide Substances

作　　者：吴艳清[1] 蒋继志[1] 梁廷银[1] 

机构地区：[1]河北大学生命科学学院,河北保定071002

出　　处：《安徽农业科学》2012年第14期8111-8112,8135,共3页Journal of Anhui Agricultural Sciences

基　　金：河北省自然科学基金资助项目(C2011201003)

摘　　要：[目的]筛选致病疫霉[Phytophthora infestans(Mont.)de Bary]拮抗芽孢杆菌并对其脂肽类物质进行分析。[方法]采用平板分离培养法从狗尾草[Setaria viridis(L.)Beauv]根际土壤中分离产芽孢细菌,采用对峙培养法对其进行初筛和复筛,并对其中杀菌效果较好的Y-3菌株的脂肽类物质进行了HPLC分析。[结果]初筛有9株细菌对P.infestans具有一定的拮抗作用,复筛得到Y-1和Y-3菌株对P.infestans的生长具有明显的抑制作用,抑菌率达80%以上;Y-3菌株产生的脂肽粗提物对P.infestans具有较好的拮抗作用,抑制率达90%以上,HPLC分析显示其脂肽粗提物主要包括8种组分。[结论]Y-3菌株产生的脂肽类物质抑菌效果显著,在生物防治马铃薯晚疫病方面具有潜在的应用价值。[ Objective ] The paper was to screen the antagonistic Bacillus spp. strains against Phytophthora infestans （ Mont. ） de Bary and an- alyze its lipopeptide substances. [ Method ] Using flat isolation and culture method, the bacteria that could produce Bacillus were isolated from rhizosphere soil, and carried out primary and secondary screening by confrontation culture. The lipopeptide substances of Y-3 strain with better antifungal effect were carried out HPLC analysis. [ Result] Nine strains of bacteria had certain antagonistic effects against P. infestans in prima- ry screening, Y-1 and Y-3 strains had significant inhibition effect against the growth of P. infestans in secondary screening, with inhibition rate higher than 80% ; the crude extract of lipopeptide produced by Y-3 strain had better antagonistic effect against P. infestans ,with inhibition rate higher than 90%. HPLC analysis showed that the crude extract of lipopeptide mainly contained 8 components. [ Conclusion ] The lipopeptide substances produced by Y-3 strain had significant inhibition effect, which had potential application value for biological control of potato late blight.

关 键 词：致病疫霉 拮抗菌 芽孢杆菌 脂肽 生物防治 

分 类 号：S436.412[农业科学—农业昆虫与害虫防治]