车前子多糖抗炎作用机制的实验研究  

Studies on Anti-Inflammatory Mechanisms of Plantain Seed Polysaccharides

作　　者：冯娜[1] 刘芳[1] 郭会彩[1] 曹阿芳[1] 王素敏[1] 

机构地区：[1]省部共建神经和血管生物学实验室,河北省新药药理毒理研究重点实验室,河北医科大学基础医学院药理教研室,石家庄050011

出　　处：《天津医药》2012年第6期598-601,共4页Tianjin Medical Journal

基　　金：河北省重点科研项目(20090314);河北省中医药管理局项目(2010078)

摘　　要：目的:观察不同浓度车前子多糖(PSP)对各期炎症模型的影响,探讨其可能机制。方法:将小鼠和大鼠随机各分为阴性对照组、阳性对照组和PSP低、中、高剂量组,每组10只。采用二甲苯致耳廓肿胀、醋酸诱发小鼠腹腔毛细血管通透性增高,蛋清致大鼠背部气囊滑膜炎,棉球诱发小鼠肉芽肿,探讨PSP的抗炎作用。称体质量测定各组小鼠耳的炎症肿胀程度及肉芽增生水平,用化学方法检测各组小鼠腹腔洗涤液中的伊文思蓝含量、滑膜炎渗出液容积、渗出液中的白细胞(WBC)计数和肿瘤坏死因子(TNF)-α含量,渗出液和血清中超氧化物歧化酶(SOD)、丙二醛(MDA)含量。结果:与阴性对照组相比,车前子多糖能够抑制二甲苯致小鼠耳廓肿胀、醋酸致小鼠毛细血管通透性的增加,以及小鼠棉球肉芽肿的形成差异有统计学意义(P<0.01);其较阴性对照组还能明显减少渗出液容积,降低渗出液中WBC、MDA、TNF-α含量及血清中MDA水平,并能提高渗出液和血清中SOD的活性,差异有统计学意义(P<0.05或P<0.01)结论:PSP能够减轻各期炎症形成,机制可能与抑制炎性因子的渗出、消除自由基和抑制脂质过氧化有关。Objective： To observe effects of different concentrations of the plantain seed polysaccharides （PSP） on the different period inflammatory models, and the possible mechanism thereof. Methods： The mice or rats were randomly divided into 5 groups, negative control group, positive control group, low-dose-PSP group, medium-dose-PSP and high-dose-PSP group （n=10 for each group）. The anti-inflammatory models of PSP were investigated in mice by methods of the xylene-in- duced auricle swelling （XIAS）, intraperitoneal injection of acetic acid-induced peritoneal capillary permeability increase （AIPCPI）, the cotton-pellet induced-granuloma（CPIG） and egg white-induced balloon back synovial inflammation（EIBBSI）. Levels of mouse XIAS and CPIG were detected by weighing respectively. Levels of mouse peritoneal washing fluid in the con- tent of Evans blue, synovitis exudate volume, white blood cells （WBC）, tumor necrosis factor （TNF-α）, the exudate and serum levels of superoxide dismutase （SOD） and malondialdehyde （MDA） were determined by chemical method respectively. Re- sults： Compared with negative control group, levels of XIAS, AIPCPI and CPIG were decreased significantly in PSP group （P 〈 0.01）. Levels of WBC, MDA and TNF-a were significantly decreased in PSP group than those of negative control group, but the activity of the exudate and serum SOD was increased significantly in PSP group compared with that of negative control group （P 〈 0.05 or P 〈 0.01）. Conclusion ： The PSP showed protective effects on inflammation in animal models, which may be mediated through inhibition of the exudation of inflammatory factors, the lipid peroxidation and the elimination of free radi- cals.

关 键 词：抗感染药 丙二醛 超氧化物歧化酶 肿瘤坏死因子Α 白细胞计数 药理作用分子作用机制 疾病模型 动物 小鼠 车前子多糖 

分 类 号：R971.2[医药卫生—药品]