吡格列酮对糖尿病大鼠肝脏chemerin、cmklr1及TNF-αmRNA表达的影响  

Effect of Pioglitazone on Expression of Chemerin,Cmklr1 and Tumor Necrosis Factor-alpha mRNA in Liver of Diabetic Rat Model

作　　者：李国琪[1] 于倩[1] 张捷[1] 刘德敏[1] 

机构地区：[1]天津医科大学代谢病医院,300070

出　　处：《天津医药》2012年第6期602-604,I0001,共4页Tianjin Medical Journal

基　　金：天津市卫生局科技基金资助项目(项目编号:09KY24)

摘　　要：目的:观察噻唑烷二酮类药物对糖尿病大鼠肝脏chemerin、cmklr1及肿瘤坏死因子(TNF)-αmRNA表达的影响。方法:雄性SD大鼠随机分为正常组、糖尿病组与吡格列酮组,尾静脉注射链脲佐菌素制作糖尿病大鼠模型,造模成功后吡格列酮组每天按15mg/kg经胃灌药,连续给药8周。第8周末处死大鼠留取肝脏组织。采用实时定量PCR法检测大鼠肝脏chemerin、cmklr1及TNF-αmRNA的表达水平。结果:糖尿病组肝脏chemerin表达低于正常组及吡格列酮组,差异均有统计学意义(均P<0.017)。3组间肝脏cmklr1表达差异无统计学意义。糖尿病组肝脏TNF-α表达较正常组升高(P<0.017),吡格列酮组较糖尿病组有所降低,但差异无统计学意义。结论:糖尿病大鼠肝脏TNF-α表达增高,吡格列酮可能通过上调chemerin的表达而改善肝脏的胰岛素敏感性。Objective： To investigate the effect of thiazolidinediones on expression of chemerin, cmklrland tumor necrosis factor （TNF）-alpha mRNA in liver of diabetic rat model. Methods： Male SD rats were randomized into three groups, normal con- trol group, diabetes group and pioglitazone group. Murine diabetic models were induced with streptozotocin （STZ）. After success- ful modeling, pioglitazone [15 mg/（kg, d）] was given to rats of pioglitazone group for 8 weeks. Rats were sacrificed to get liver tis- sues at the end of 8 weeks. Expressions of chemerin, cmklrl and TNF-α mRNA were detected by the method of real-time quanti- tative PCR. Results： Results of real-time quantitative PCR showed that expression of chemerin mRNA was significantly lower in diabetes group than that of normal control group and pioglitazone group （P 〈 0.017）. NO significant difference was found in ex- pression of cmklrl mRNA between three groups. The expression of TNF-α mRNA was significantly higher in diabetes group than that of normal control group （P 〈 0.017）. Conclusion： The expression of TNF-α mRNA was upregulated in the liver tissue of diabetes group. Pioglitazone may improve insulin sensitivity of liver by upregulating expression of chemerin mRNA.

关 键 词：糖尿病 实验性 肝 肿瘤坏死因子Α 大鼠 Sprague-Dawley 吡格列酮 CHEMERIN cmklr1 

分 类 号：R446.61[医药卫生—诊断学]