大肠埃希菌生物被膜形成能力及超广谱β-内酰胺酶检测  

Detection of the biofilm formation ability and the extended spectrum β-lactamase of Escherichia coli clinical isolates

作　　者：刘丹[1] 刘晓峰[1] 郎梅春 潘志雄[1] 陈越[1] 

机构地区：[1]江西省九江市第一人民医院检验科,江西九江332000 [2]江西省九江市第三人民医院检验科,江西九江332000

出　　处：《实验与检验医学》2012年第2期140-142,共3页Experimental and Laboratory Medicine

基　　金：江西省九江市科委市级科研课题项目编号九科字(2011)67号

摘　　要：目的了解大肠埃希菌临床分离株生物被膜的形成能力及对常用抗菌药物的耐药特性,以便指导临床更合理使用抗菌药物。方法半定量微量板法检测389株大肠埃希菌临床分离株生物被膜形成情况。采用表型确证试验检测超广谱β-内酰胺酶(ESBLs),药敏试验采用K-B法。结果389株大肠埃希菌中有301株生物被膜形成阳性,阳性率为77.4%,其中绝大部分形成中等或较大量的生物被膜;能形成生物被膜的大肠埃希菌中产ESBLs酶占53.2%(160/301);产酶的生物被膜菌对12种抗菌药物(除亚胺培南全部敏感外)的耐药率均较高。结论大肠埃希菌临床分离株生物被膜形成能力非常强,耐药及多重耐药性较严重。Objective To understand the biofilm-formation ability and drug resistance characteristics of Escherichia coli clinical isolates,in order to provide a theoretical basis for the clinical application of antibiotics.Methods The method of microtiterplate culture-crystal violet stain was established for the quantificative analysis of the biofilm-formation of 389 Escherichia coli isolates.Extended spectrum β-lactamases（ESBLs） was detected by phenotypic confirmatory test and K-B method was used for susceptibility test.Results Among 389 Escherichia coli isolates examined,301 of them formed biofilm,the positive rate was 77.4%,most of the Escherichia coli isolates formed the middle quantity or a large number of biofilm;160 bacteria produced Extended spectrum β-lactamases among 301 Escherichia coli biofilm bacteria,accounting for 53.2%;the biofilm bacteria producing extended spectrum β-lactamases had higher drug resistance rate to 12 antibiotics except imipenem.Conclusion Escherichia coli clinical isolates have very strong biofilm formation ability.The drug resistance and multidrug resistance of these isolates are more serious.

关 键 词：大肠埃希菌 细菌生物被膜 形成能力 超广谱Β-内酰胺酶 

分 类 号：R446.5[医药卫生—诊断学] Q939.92[医药卫生—临床医学]