干细胞特异性标志物Oct-4在表皮肿瘤中表达的临床实验研究  

Oct-4 expression in epidermal tumors

作　　者：沈颖蔡 绥勃[2] SHEN Ying;CAI Sui—qin(Department of Dermatology,Zhuji People's Hospital of Zhejiang Province,Zhuji 311800,China;不详)

机构地区：[1]诸暨市人民医院皮肤科,浙江省诸暨311800 [2]浙江大学医学院附属二院皮肤性病科,浙江省诸暨311800

出　　处：《中国医师杂志》2012年第5期585-588,共4页Journal of Chinese Physician

摘　　要：目的探讨表皮非黑素肿瘤中干细胞特异性标志物Oct-4的表达情况以及在表皮肿瘤发生中的作用。方法本研究选择20例人皮肤鳞状细胞癌(scc)、20例基底细胞癌(BCC)、20例脂溢性角化(SK)和20例正常人皮肤组织,采用EnVision免疫组化检测各组组织中干细胞特异性标志物Oct-4和增殖细胞核抗原(PCNA)的表达情况,分析其表达意义。结果(1)Oct-4在皮肤SCC、BCC中表达强度分别为90%(18/20)、80%(16/20),与正常皮肤组织65%(13/20)和皮肤SK75%(15/20)相比明显增高(P〈0.05),Oct-4在皮肤SCC和BCC之间的表达比较差异无统计学意义(P〉0.05)。Oct-4在皮肤SK和正常皮肤组织之间的表达比较差异无统计学意义(P〉0.05)。Oct-4在皮肤SK和BCC之间的表达比较差异无统计学意义(P〉0.05)。(2)PCNA在皮肤SCC、BCC中表达强度分别为95%(19/20)、90%(18/20),与正常皮肤组织75%(12/20)和皮肤SK85%(17/20)比较差异有统计学意义(P〈0.01);在皮肤SCC和BCC之间的表达比较差异有统计学意义(P〈0.05);在皮肤SK和正常皮肤组织之间的表达比较差异有统计学意义(P〈0.05);而PCNA在皮肤SK和BCC的表达比较差异无统计学意义(P〉0.05)。(3)Oct-4在皮肤SCC、BCC中表达强度与细胞周期蛋白PCNA表达阳性强度均呈正相关(r=0.450,0.457,P〈0.05)。结论干细胞特异性标志物Oct-4在BCC和SCC中表达强度明显增高,可能与BCC和SCC发生发展有关。PCNA在BCC和SCC中强阳性表达,可能参与了BCC和SCC增殖过程。Oct-4和PCNA在皮肤SCC、BCC中的阳性表达强度呈正相关性,Oct-4可能通过影响细胞增殖参与了BCC和SCC的发病过程。Objective The aim of this study was to understand the role of specific markers of stem cells Oct-4 expression in the development of human epidermal non-melanoma cutaneous tumors.Methods The paraffin-embedded samples were retrieved from files of pathology department at our hospital,including 20 cases of skin squamous cell carcinomas(SCC),20 cases of basal cell carcinomas(BCC),20 cases of seborrhoeic keratosis(SK)and 20 cases of normal skin(from head,face,trunk,extremities).The ex-pression of Oct-4 and PCNA were observed by immunohistochemical staining technique.Results Oct-4 protein was abnormally increased in SCC and BCC compared to normal skin and SK(P〈0.05).However there were no significant difference of Oct-4 protein expression between SCC and BCC(P〉0.05).There were also no significantly different Oet-~protein expression between Sk and the normal skin(P〉O.05),and no significantly different Oct-4 protein expression between SK and BCC(P〉0.05).PCNA protein was abnormally increased in SCC and BCC compared to normal skin and SK(P〈0.01).There were significantly different PCNA protein expression between SCC and BCC(P〈0.05).There were also significantly different PCNA protein expression between SK and the normal skin(P〈0.05).However there were no significant difference of PCNA protein expression between SK and BCC(P〉0.05).There were positive correlation between the expression intensity of Oct-4 and PCNA in SCC and BCC.Conclusions The abnormal expression of Oct-4 may have an important role in the development of BCC and SCC.Positive Oct-4 expression cells may be the tumor stem cell in SCC and BCC.There were positive correlation between the expression intensity of Oct-4 and PCNA in SCC and BCC.The over expression Oct-4 in BCC and SCC may play an important role in proliferation of tumor.

关 键 词：八聚体转录因子类/代谢 癌 鳞状细胞/代谢/病理学 癌 基底细胞/代谢/病理学 角化病 脂溢性/代谢/病理学 

分 类 号：R331.4[医药卫生—人体生理学]