通心络对缺血再灌注损伤大鼠脑组织细胞因子EPOmRNA变化的影响  

Effects of Tongxinluo on cytokine EPO mRNA of rats brain after ischemical reperfusion injury

作　　者：卢昌均[1] 鹿俊磊[1] 安红伟[1] 刘国成[1] 周哲屹[1] 韦冰心[1] 陆兵勋[2] 王立新[3] 

机构地区：[1]广西柳州市中医院神经内科,545001 [2]南方医科大学神经内科 [3]广东省中医院脑病科

出　　处：《国际中医中药杂志》2012年第6期516-518,共3页International Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目（项目编号：NO.30400612）

摘　　要：目的探讨促红细胞生成素（EPO）mRNA在缺血／再灌注损伤大鼠脑组织的表达情况以及通心络对EPOmRNA的影响。方法采用大鼠缺血／再灌注损伤（MCAO）模型并予通心络灌胃，应用逆转录集合酶链式反应（RT-PCR）检测缺血再灌注损伤后3、5、7、14d神经干细胞增殖分化相关细胞因子EPOmRNA的变化。结果缺血再灌注模型组EPOmRNA在不同时间段均有表达，EPOmRNA从造模后第3天表达增强，第5天表达最高；给予通心络处理后，第3天开始，缺血侧EPOmRNA表达增强，第5、7、14天PCR表达灰度值均高于模型组。结论大鼠脑缺血再灌注后，缺血区脑组织细胞因子EPOmRNA表达增强，通心络可强化其反应，进而可能诱导神经于细胞增殖和分化。Objective To explore the expression of hemopoietin（EPO） mRNA on cerebral ischemia-reperfusion injury in rats brain tissue and the effect of Tongxinluo on it. Methods The model of rat （MCAO） were perfused with Tongxinluo, the changes of neural stem cell proliferation and differentiation related cell factors of EPO mRNA were detected after ischemia-reperfusion injury 3、 5、7、 14 d by means of reverse transcriptase polymerase chain reaetion（RT-PCR）. Results EPO mRNA of ischemia-reperfusion models showed expression in different period, the expression enhanced in the third day, reached the highest in the fifth day; the ischemia side EPO mRNA expression enhanced in the third day after give Tongxinluo, in the 5, 7 and 14 day, PCR expression gray values were higher than the model group. Conclusion EPO mRNA expression enhanced after cerebral ischemia, this expression can be strengthened by Tongxinluo, and may further induce neural stem cell proliferation and differentiation.

关 键 词：脑缺血 促红细胞生成素mRNA 通心络 

分 类 号：R285.5[医药卫生—中药学]