基质细胞衍生因子1对骨关节炎软骨Ⅱ型胶原及分泌基质金属蛋白酶的影响  

Effect of stromal cell derived factor-1 on collagen type Ⅱ and matrix metalloproteinase secretion in osteoarthritis

作　　者：王国梁[1] 李彦林[1] 高岗[1] 马珂[1] 陈文栋[1] 许鹏[1] 杨光[1] 

机构地区：[1]昆明医学院第一附属医院骨科,云南省昆明市650031

出　　处：《中国组织工程研究》2012年第20期3639-3643,共5页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金资助项目(30860286)~~

摘　　要：背景:基质细胞衍生因子1/趋化因子受体4信号通路在骨关节炎的发病中起关键作用。目的:观察外源性基质细胞衍生因子1对骨关节炎软骨Ⅱ型胶原及分泌基质金属蛋白酶的影响。方法:分别取因骨关节炎行膝关节置换患者软骨组织块40块,作为实验组;取因创伤性截肢患者膝关节的正常软骨块40块,作为对照组;置入DMEM培养液中,加入79,392μg/L外源性基质细胞衍生因子1,分别培养48h和96h。结果与结论:实验组与对照组培养48,96h后,各时间段Ⅱ型胶原免疫组织化学染色皆呈阳性,但高浓度基质细胞衍生因子1培养液条件下时间越长Ⅱ型胶原降解越严重;实验组与对照组同一时间段同浓度基质细胞衍生因子1培养液条件下基质细胞衍生因子1水平无明显差异(P>0.05);高浓度基质细胞衍生因子1培养液条件下同一时间段实验组比对照组中促使软骨释放基质金属蛋白酶多(P<0.05)。提示基质细胞衍生因子1可通过基质细胞衍生因子1/趋化因子受体4信号通路调节骨关节炎软骨基质金属蛋白酶表达并致软骨Ⅱ型胶原降解。BACKGROUND： The stromal cell derived factor-1 （SDF-1）/chemokine receptor 4 （CXCR4） signal pathway plays a key role in the pathogenesis of osteoarthritis. OBJECTIVE： To study the effect of SDF-1 on collagen type Ⅱ and matrix metalloproteinase （MMPs） secretion in osteoarthritis. METHODS： Forty knee cartilage blocks from osteoarthritis patients who had total knee replacement were selected as experimental group. Forty knee cartilage blocks from patients who had traumatic amputation were as the control group. The samples were put in Dulbecco＇s modified Eagle’s medium and cultivated for 48 and 96 hours with 79 and 392 μg/L exogenous SDF-1. RESULTS AND CONCLUSION： After cultured for 48 and 96 hours, the expressions of collagen type Ⅱ were both positive in the two groups by immunohistochemistry staining; the collagen type Ⅱ degenerated more when it was exposed in high-concentration SDF-1 culture medium with the longer time. There was no significant difference of SDF-1 in SDF-1 culture medium at the same time and concentration between the experimental and control groups （P﹥0.05）. The MMPs of the experimental group was released more than that of the control group in high-concentration SDF-1 culture medium （P 〈 0.05）. These showed that SDF-1 could regulate the expression of MMPs and lead to the degradation of collagen type Ⅱ through SDF-1/CXCR4 signal pathways in osteoarthritis.

关 键 词：基质细胞衍生因子1 Ⅱ型胶原 基质金属蛋白酶 骨关节炎 软骨 

分 类 号：R318[医药卫生—生物医学工程]