张应力刺激大鼠髁突软骨细胞Ⅱ型胶原和聚集蛋白聚糖的表达(英文)  

Effect of tensile stress on type Ⅱ collagen and aggrecan expression in rat condylar chondrocytes

作　　者：郑如松[1] 杨竹丽 杜衍晓 尹崇英[1] 贾萍萍[1] 袁晓 

机构地区：[1]青岛大学医学院附属青岛市立医疗集团口腔科,山东省青岛市266000 [2]青岛市市立医疗集团口腔科,山东省青岛市266071 [3]青岛市中心医院,山东省青岛市266042

出　　处：《中国组织工程研究》2012年第20期3649-3653,共5页Chinese Journal of Tissue Engineering Research

基　　金：the National Natural Science Foundation of China,No.31170891~~

摘　　要：背景:研究表明软骨细胞外基质合成的变化反映了外力对于颞下颌关节的影响和机体对于外力的适应性。目的:观察周期性张应力对髁突软骨细胞主要细胞外基质合成的影响。方法:采用FX-5000T应力加载系统对第3代大鼠髁突软骨细胞分别施加0,1,6,12和24h的周期性张应力,应力刺激强度为10%1Hz。加力完成后收集加力细胞,提取总RNA反转录成cDNA,应用RT-PCR技术检测软骨细胞主要细胞外基质Ⅱ型胶原和聚集蛋白聚糖的表达变化情况。结果与结论:与对照组(0h组)相比,加力6h时Ⅱ型胶原和聚集蛋白聚糖表达均显著增加(P<0.05);加力12h时Ⅱ型胶原和聚集蛋白聚糖表达均开始下降;当加力至24h时二者表达量均显著降低(P<0.05)。结果表明:周期性张应力可以影响髁突软骨细胞主要细胞外基质的合成,随加力时间的延长基质合成逐渐增强;进一步延长加力时间,基质的合成受到明显抑制。BACKGROUND： Changes in extracellular of chondrocyte can reflect influence of external force on temporomandibular joint and adaptability of body to external force. OBJECTIVE： To study the effect of cyclic tensile stress on main extracellular matrix of condylar chondrocyte. METHODS： The cyclic tensile stress was exposed to the third passage condylar chondrocyte for 0, 1, 6, 12 and 24 hours, respectively, using a Flexcell Strain Unit-5000T system （10% surface elongation, 6 cycles/min）. After mechanical loading, total RNA was extracted from the cells harvested from Six-well BioFlex flexible cell Petri Dish, reverse transcribed, and reverse trabscription-PCR was performed to quantify mRNA levels for type Ⅱ collagen and aggrecan. RESULTS AND CONCLUSION： Compared with the control group （0 hour group）, both type Ⅱ collagen and aggrecan mRNA expression was significantly increased after loading for 6 hours （P 〈 0.05）, but began to decrease since 12 hours, and significantly decreased at 24 hours （P 〈 0.05）. Results showed that cyclical tensile stress stimuli can affect the synthesis of main extracellular matrix of condylar cartilage, i.e. the synthesis was gradually enhanced with prolonged stimulation duration, but significantly inhibited in response to further stress stimuli.

关 键 词：张应力 功能矫形 髁状突软骨 Ⅱ型胶原 聚集蛋白聚糖 

分 类 号：R318[医药卫生—生物医学工程]