高脂饮食诱导肥胖模型大鼠骨骼肌组织蛋白酪氨酸磷酸酯酶1B和胰岛素受体底物2的表达  

Expression of protein-tyrosine phosphatase 1B and insulin receptor substrate 2 in the skeletal muscle of rats with insulin resistance induced by high-fat diet

作　　者：赵慧[1] 于苏国[1] 王令令[2] 赵艳敏[1] 

机构地区：[1]滨州医学院附属医院内分泌科,山东省滨州市256603 [2]滨州市人民医院,山东省滨州市256600

出　　处：《中国组织工程研究》2012年第20期3698-3702,共5页Chinese Journal of Tissue Engineering Research

摘　　要：背景:外周组织的胰岛素抵抗是2型糖尿病的主要病因。目的:观察高脂饮食诱导的肥胖大鼠骨骼肌中蛋白酪氨酸磷酸酯酶1B和胰岛素受体底物2的表达。方法:将20只SD大鼠随机等分为对照组和高脂组,分别给予常规饲料和高脂饲料喂养12周。结果和结论:与对照组相比,高脂组大鼠胰岛素敏感指数显著降低(P<0.01),大鼠葡萄糖耐量受损,胰岛素释放试验提示葡萄糖刺激的胰岛素第一时相分泌受损,骨骼肌组织中蛋白酪氨酸磷酸酯酶1B蛋白表达水平明显增加(P<0.01),骨骼肌中胰岛素诱导的胰岛素受体底物2磷酸化程度降低(P<0.01)。提示高脂饮食诱导的肥胖大鼠骨骼肌中蛋白酪氨酸磷酸酯酶1B蛋白表达量升高,使胰岛素诱导的胰岛素受体底物2磷酸化程度降低,可能是肥胖导致胰岛素抵抗的机制之一。BACKGROUND： Insulin resistance in the peripheral tissue is a major cause of type 2 diabetes mellitus. OBJECTIVE： To observe the expression of protein tyrosine phosphatase 1B （PTP-1B） and insulin receptor substrate 2 （IRS-2） in the skeletal muscle of rats with insulin resistance induced by high-fat diet. METHODS： Twenty SD rats were randomly divided into normal control group with 10 rats and high-fat diet group with 10 rats. Rats in the two groups were fed with normal diet and high-fat diet for 12 weeks, respectively. RESULTS AND CONCLUSION： The insulin sensitive index was significantly decreased in the high-fat diet rats compared with the normal rats （P 〈 0.01）. In obese rats, glucose tolerance and the acute first-phase insulin secretory response to glucose were impaired. The protein level of PTP-1B in the skeletal muscle of obese rats was significantly increased compared with the control group （P 〈 0.01）. Insulin-stimulated IRS-2 phosphorylation in the skeletal muscle was reduced in the obese rats （P 〈 0.01）. These indicate that the increased PTP-1B level and the reduced insulin-stimulated IRS-2 phosphorylations in the skeletal muscle seem to play an important role in the insulin resistance induced by high-fat diet.

关 键 词：肥胖 蛋白酪氨酸磷酸酶1B 胰岛素受体底物2 骨骼肌 胰岛素抵抗 

分 类 号：R318[医药卫生—生物医学工程]