选择性深低温血流阻断猴海马超微结构和波形蛋白表达的变化  

Effects of selective profound hypothermia on the ultrastructure and Vimentin expression of monkey cerebral hippocampi after severe cerebral ischemia

作　　者：牛小群[1] 范耀东[2] 蒲军[3] 李疆晓[4] 高永军[3] 付国平[3] 方绍龙[3] 徐蔚[3] 

机构地区：[1]昆明医学院第二附属医院呼吸内科 [2]昆明医学院第三附属医院神经外科,云南省昆明市650118 [3]昆明医学院第二附属医院神经外科,云南省昆明市650101 [4]昆明医学院第二附属医院石林天奇医院神经外科,云南省昆明市652200

出　　处：《中国组织工程研究》2012年第20期3707-3710,共4页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金资助项目(30960398);云南省自然科学基金资助项目(2009cd178)~~

摘　　要：背景:前期研究显示,选择性深低温顺行脑灌注能提高猴脑对缺血缺氧的耐受性。目的:观察选择性深低温血流阻断对猴海马超微结构和波形蛋白表达的影响。方法:将8只健康成年恒河猴随机分为两组:深低温组(n=5)在常温下阻断猴双侧颈总动脉及颈内静脉10min,然后通过一侧颈内动脉灌注4℃林格氏液,同侧颈内静脉回流,使脑温维持在18℃以下,60min后恢复脑血流;常温组(n=3)除灌注液改为37℃林格氏液外,其余与深低温组相同。结果与结论:深低温组猴全部成功复苏,海马组织形态及超微结构未见明显异常。常温组猴全部死亡,未能成功复苏,海马组织形态及超微结构有不同程度异常。与常温组比较,深低温组海马波形蛋白表达显著降低(P<0.01)。说明阻断双侧颈总动脉10min后行选择性深低温顺行脑灌注可下调海马波形蛋白表达,保护海马组织,减轻脑缺血损害。BACKGROUND： Previous studies have shown that cerebral selective profound hypothermia combined with antegrade cerebral perfusion can improve the resistance of monkeys to cerebral hypoxia-ischemia. OBJECTIVE： To observe the effect of selective cerebral profound hypothermia on the ultrastructure and Vimentin expression of monkey hippocampi after severe cerebral ischemia. METHODS： Eight healthy adult rhesus monkeys were randomly divided into two groups： profound hypothermia （n=5）, and normothermia （n=3）. For the monkeys in the profound hypothermia group, bilateral carotid arteries and jugular veins were occluded for 10 minutes at room temperature; Ringer’s solution at 4 ℃ was then perfused through the right internal carotid artery and flowed out of the right jugular vein, and the brain temperature remained below 18 ℃; 60 minutes later, the cerebral blood flow was restored. For the normothermia group, all procedures were the same except that the perfusion liquid was replaced with Ringer＇s solution at 37 ℃. RESULTS AND CONCLUSION： All animals in the profound hypothermia group were successfully resuscitated. No significant abnormalities of the hippocampus morphology and ultrastructure were observed. In contrast, in the normothermia group, no monkeys were alive after perfusion, with abnormal hippocampus morphology and ultrastructure to different extents. Vimentin expression in the hippocampus was significantly lower in the profound hypothermia group than the normothermia group （P 〈 0.01）. Selective cerebral profound hypothermia following 10-minute occlusion of the bilateral common carotid arteries could down-regulate Vimentin expression in the hippocampus and protect the hippocampus from severe cerebral ischemia.

关 键 词：选择性深低温 脑缺血 海马 超微结构 波形蛋白 

分 类 号：R318[医药卫生—生物医学工程]