银杏叶提取物对胚胎大鼠中脑原代细胞及多巴胺能神经元活性的影响  

Effect of ginkgo biloba extract on activities of primary cells from embryonic rat mesencephalon and dopaminergic neurons

作　　者：奚淑芳[1] 王群江[2] 朱灿宏[1] 胡蓉[1] 端礼荣[3] 

机构地区：[1]镇江市第一人民医院,江苏省镇江市212002 [2]浙江省人民医院,浙江省杭州市310051 [3]江苏大学医学院预防医学系,江苏省镇江市212001

出　　处：《中国组织工程研究》2012年第20期3751-3754,共4页Chinese Journal of Tissue Engineering Research

基　　金：2006年江苏省镇江市科技计划项目基金资助(SH2006043);项目名称:银杏叶提取物对大鼠中脑原代细胞的作用~~

摘　　要：背景:银杏叶提取物具有清除自由基和过氧化脂质,对抗兴奋性神经递质释放等多种药理活性。目的:观察银杏叶提取物EGB761对1-甲基-4-苯基吡啶离子(1-methyl-4-phenylpyridinum,MPP+)及左旋精氨酸(L-arginine,L-Arg)干预后的中脑原代细胞及多巴胺能神经元是否具有保护作用。方法:分离培养SD胚胎大鼠中脑神经细胞,分组培养:空白对照组、MPP+组、L-Arg组、MPP++L-Arg组、MPP++EGB761组、MPP++EGB761+L-Arg组。结果与结论:①细胞A值:MPP+组、MPP++L-Arg组显著低于空白对照组、L-Arg组、MPP++EGB761组及MPP++EGB761+L-Arg组(P<0.05),MPP++L-Arg组低于MPP+组(P<0.05),MPP++EGB761+L-Arg组低于MPP++EGB761组(P<0.05)。②酪氨酸羟化酶阳性细胞数:MPP+组、MPP++L-Arg组显著低于空白对照组、L-Arg组、MPP++EGB761组及MPP++EGB761+L-Arg组(P<0.05),MPP++L-Arg组低于MPP+组(P<0.05),MPP++EGB761+L-Arg组低于MPP++EGB761组(P<0.05)。③细胞一氧化氮水平:MPP+组、MPP++L-Arg组显著高于空白对照组、L-Arg组、MPP++EGB761组及MPP++EGB761+L-Arg组(P<0.05),MPP++L-Arg组高于MPP+组(P<0.05),MPP++EGB761+L-Arg组高于MPP++EGB761组(P<0.05)。说明L-Arg可加重MPP+对多巴胺能神经元的损伤作用,EGB761对损伤后的胚胎大鼠中脑原代培养细胞有保护作用,此作用可能是通过抑制诱导型一氧化氮合酶活性降低一氧化氮产生完成的。BACKGROUND： Ginkgo biloba extract （EGB） has the pharmacological activities of free radical and lipid peroxidation scavenging and release of excitatory neurotransmitter resisting. OBJECTIVE： To explore whether EGB761 has protective effect on primary cells from embryonic rat mesencephalon and dopaminergic neurons from injuries induced by 1-methyl-4-phenylpyridinum （MPP＋） and L-arginine （L-Arg）. METHODS： Midbrain nerve cells from Sprague Dawley embryonic rats were isolated and cultured. The cells were divided into control group, MPP＋ group, L-Arg group, MPP＋＋L-Arg group, MPP＋＋EGB761 group and MPP＋＋EGB761＋L-Arg group. RESULTS AND CONCLUSION： Comparison results of cell A values and numbers of tyrosine hydroxylase-positive cells among the groups： these indexes in the MPP＋ group and MPP＋＋L-Arg group were significantly decreased compared with the control group, L-Arg group, MPP＋＋EGB761 group and MPP＋＋EGB761＋L-Arg group （P 〈 0.05）, those of the MPP＋＋L-Arg group were lower compared with the MPP＋group （P 〈 0.05）, and those of the MPP＋＋EGB761＋L-Arg group were lower than the MPP＋＋ EGB761 group （P 〈 0.05）. Cell nitric oxide levels： the levels in the MPP＋ group and MPP＋＋L-Arg group were obviously higher compared with the control group, L-Arg group, MPP＋＋EGB761 group and MPP＋＋EGB761 L-Arg group （P 〈 0.05）, the level of the MPP＋＋L-Arg group was higher compared with the MPP＋ group （P 〈 0.05）, and that of the MPP＋＋EGB761＋L-Arg group was higher than the MPP＋＋EGB761 group （P 〈 0.05）. It is indicated that L-Arg can increase the MPP＋-induced damage to nopaminergic neurons, and EGB761 has the protective effect on injured primary cells from embryonic rat mesencephalon which may derived from reduction of nitric oxide by inhibiting the activity of inducible nitric oxide synthase.

关 键 词：银杏叶提取物 一氧化氮 左旋精氨酸 1-甲基-4-苯基吡啶离子 神经细胞 

分 类 号：R318[医药卫生—生物医学工程]