新型仿生人工神经导管诱导犬周围神经再生过程的组织学观察  

Histological observation of dog peripheral nerve regeneration induced by a novel biomimetic nerve conduit

作　　者：向杰[1,2] 黄继锋[2] 李德忠[2] 李世普[3] 闫玉华[3] 严琼姣[3] 

机构地区：[1]南方医科大学,广东省广州市510515 [2]解放军广州军区武汉总医院骨科,湖北省武汉市430070 [3]武汉理工大学生物材料与工程研究中心,湖北省武汉市430070

出　　处：《中国组织工程研究》2012年第21期3801-3805,共5页Chinese Journal of Tissue Engineering Research

基　　金：湖北省自然科学基金重点项目(2009CDA041);武汉市科技攻关计划项目(200960323125)~~

摘　　要：背景:前期动物实验已经证明,精氨酸-甘氨酸-天冬氨酸多肽接枝聚(羟基乙酸-L-赖氨酸-乳酸)/β-磷酸三钙/神经生长因子导管可以修复大鼠坐骨神经10mm缺损,该材料具有良好的生物相容性和细胞亲和性。目的:观察精氨酸-甘氨酸-天冬氨酸多肽接枝聚(羟基乙酸-L-赖氨酸-乳酸)/聚乳酸/β-磷酸三钙/神经生长因子导管诱导犬周围神经再生过程的组织学变化。方法:取健康成年杂种犬12只,建立30mm腓总神经缺损模型,用精氨酸-甘氨酸-天冬氨酸多肽接枝聚(羟基乙酸-L-赖氨酸-乳酸)/聚乳酸/β-磷酸三钙/神经生长因子人工神经导管进行桥接修复。结果与结论:修复后3个月时在神经远端即可发现新生神经纤维,随着时间的推移,再生神经轴突直径及髓鞘厚度不断增加,9个月时再生神经中可见较多发育成熟的有髓神经纤维。胫骨前肌损伤后出现逐渐萎缩,修复后6,9个月萎缩肌肉逐渐恢复。从组织学角度证实了精氨酸-甘氨酸-天冬氨酸多肽接枝聚(羟基乙酸-L-赖氨酸-乳酸)/聚乳酸/β-磷酸三钙/神经生长因子导管可修复大动物粗大神经缺损。BACKGROUND： Previous research has shown that the novel arginine-glycine-aspatic acid （RGD） peptide modification of poly{（lactic acid）-co-[（glycolic acid）-alt-（L-lysine）]}/poly（d,l-lactic acid）/β-tricalcium phosphate/nerve growth factor （PRGDIPDLLA/β-TCPINGF） composite nerve conduit can effectively repair 10-mm sciatic nerve defects of rats, while it possess good cell affinity and biocompatibility. OBJECTIVE ： To observe the histological observation of dog peripheral nerve regeneration with PRGD/PDLLA/β-TCP/NGF composite nerve conduit. METHODS： Twelve healthy hybrid dogs were used as animal models. The conduits were employed to bridge the 30 mm defects in the peroneal nerve. RESULTS AND CONCLUSION ： Newborn regenerated myelinated nerve fibers were found in the distal section at 3 months. The diameters of myelinated nerve fibers and thickness of myelin sheath were increased day by day. Tibiatis anterior muscles showed gradual atrophy after operation, which peaked 3 months after denervation. Subsequently, the atrophy of tibialis anterior muscles recovered slowly at 6 and 9 months. This study proves the feasibility of the PRGD/PDLLA/β-TCP/NGF nerve conduit for repairing large-diameter nerve defects in large animals.

关 键 词：神经导管 神经生长因子 聚乳酸 Β-磷酸三钙 周围神经损伤 神经再生 

分 类 号：R318[医药卫生—生物医学工程]