羟基丁酸-羟基辛酸共聚物/脱细胞软骨基质复合支架的制备与体外降解  

Preparation and in vitro degradation of poly(hydroxybutyrate-co-hydroxyoctanoate) /acellular cartilage matrix composite scaffolds

作　　者：张鹏[1] 张永红[1] 

机构地区：[1]山西医科大学附属第二医院骨创科,山西省太原市030001

出　　处：《中国组织工程研究》2012年第21期3811-3814,共4页Chinese Journal of Tissue Engineering Research

基　　金：国家自然基金主任项目(31040030);新型多聚羟基烷酸软骨组织工程载体的研究;时间为2011-01/12~~

摘　　要：背景:羟基丁酸-羟基辛酸共聚物具有高生物相容性和降解性,但单一材料无法满足组织工程支架的要求。脱细胞软骨基质是制备复合支架的常用材料,具有良好的生物相容性和无抗原性等优点。目的:将羟基丁酸-羟基辛酸共聚物、脱细胞软骨基质以不同比例混合制备复合支架,观察其体外降解速率。方法:取新鲜猪关节软骨,用含有双抗的PBS液浸泡,放入含有苯甲基磺酰氟的tri-HCl缓冲液,加入DNase酶和RNase酶,用D-Hank’s液冲洗等步骤制备脱细胞软骨基质。采用溶剂浇注-颗粒沥滤的方法与羟基丁酸-羟基辛酸共聚物按不同浓度混合,制备出不同比例的复合支架。结果与结论:脱细胞软骨基质的比例不同,复合材料的完全降解时间也不同,8%含量的脱细胞软骨基质最符合软骨组织工程支架的要求。BACKGROUND： Poly （hydroxybutyrate-co-hydroxyoctanoate） （PHBHOx） has good biological characteristics, high biocompatibility and biodegradability. But the single material cannot satisfy the requirements of tissue engineering composite scaffolds. Acellular cartilag matrix （ACM） is commonly used in the preparation of composite scaffolds, and has excellent biocompatibility and no antigenicity. OBJECTIVE： To prepare the composite scaffolds and to observe the degradation rate of PHBHOx/ACM in vitro. METHODS： The articular cartilage from pigs were immersed in phosphate buffered saline containing double antioxidant, then cultured in tris hydroxymethyl aminomethane containing phenylmethanesulfony fluoride, digested with deoxyribonuclease and ribonuclease and rinsed with D-Hank＇s solution to prepare ACM samples. Solvent casting-particle leaching method was used to prepare composite scaffolds with different ratios of ACM and PHBHOx. RESULTS AND CONCLUSION： In different proportion of PHBHOx/ACM composite scaffolds, 8% ACM accords with requirements for cartilage tissue engineering scaffolds.

关 键 词：羟基丁酸-羟基辛酸共聚物 脱细胞软骨基质 复合支架 体外降解速率 软骨组织工程 

分 类 号：R318[医药卫生—生物医学工程]