基于三维CT图像辅助研制表面纳米化的仿生椎间融合器  

Design of a biomimetic nanoscaled spinal cage by using computed tomography based three-dimensional construction

作　　者：郭洪刚[1] 刘静[2] 李峰坦[1] 杨少光 陈治[4] 姚芳莲[5] 代凤英[6] 刘文广[6] 

机构地区：[1]天津医科大学总医院骨科,天津市300052 [2]天津市中心妇产科医院,天津市300052 [3]中国医学科学研究院血液病研究所,天津市300052 [4]天津大学精密学院,天津市300072 [5]天津大学化工学院,天津市300072 [6]天津大学材料学院,天津市300072

出　　处：《中国组织工程研究》2012年第21期3851-3854,共4页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(81041061);天津市教委科技基金资助项目(20090125)~~

摘　　要：背景:椎间融合器的表面性征是制约其远期临床疗效的关键因素。如何优化可降解性融合器的生物活性和结构特征以适应细胞生长是仿生研制椎间融合器的核心步骤。目的:分析数字化影像学及表面修饰技术研制椎间支架的可行性,评估构筑生物支架的方法。方法:获取颈椎标本解剖数据,对相邻层匹配轮廓间三维表面进行重构来确定支架形貌,以Nd:YAG激光联合RGD表面修饰纳米级β-磷酸三钙/壳聚糖/聚己内酯支架,观察支架形貌,测定支架的相容性、亲水性及降解力学特性。结果与结论:图像三维重构可提高支架外部轮廓的精确度,减少单纯理化制备过程的参数误差,使支架空间三维布局更加合理,联合表面改性后的支架拥有稳定的降解速率及良好的亲水表面,能达到椎间支架的力学要求,且具备良好的生物相容性,是一种理想的椎间融合器候选材料。BACKGROUND： The surface characteristics of nanoscaled spinal cage are the key factors to restrict the long-term clinical efficacy. The key step to develop the nanoscaled spinal cage is how to optimize the biological activity and structural features of the biodegradable cage to meet the cell growth. OBJECTIVE： To analyze the feasibility of digital imaging and surface-modification in developing the spinal cage and to evaluate the method to construct the scaffold. METHODS： The morphology of the scaffold was obtained by analyzing anatomic data from digital scanning in spinal area; the biomimetic nanoscaled spinal cage was made with combination of 6-tricalcium phosphate/chitosan/polycarpolactone via Nd： YAG with RGD modification. Morphology of the scaffold was observed, and its compatibility, hydrophilicity and degradation and biomechanic characteristics were observed at different periods. RESULTS AND CONCLUSION： The three-dimensional construction of imaging could improve the fabrication accuracy of outer morphology for the scaffold, and it could reduce the parameter error during the simple physical and chemical preparation process which made the physical layout of the scaffold was remarkably arranged. As a satisfactory candidate for the spinal cage, Nd： YAG plus RGD surfaced modified scaffold showed more stable chemophysical characteristics and excellent affinity, and can meet the mechanical requirements of cage scaffolds. It has a good biocompatibility and has been regarded as an ideal candidate material for spinal cages.

关 键 词：仿生椎间融合器 图像 三维重建 纳米 融合器 表面改性 

分 类 号：R318[医药卫生—生物医学工程]