钙磷涂层镁合金植入体周围组织中骨形成蛋白2的表达  

Bone morphogenetic protein-2 expression in the surrounding tissues of calcium phosphate coating magnesium alloys

作　　者：孙伟[1] 张广道[1] 谭丽丽[2] 杨柯[2] 洪岩松[1] 艾红军[1] 

机构地区：[1]中国医科大学口腔医学院口腔修复科,辽宁省沈阳市110002 [2]中国科学院金属研究所,辽宁省沈阳市110016

出　　处：《中国组织工程研究》2012年第21期3907-3910,共4页Chinese Journal of Tissue Engineering Research

基　　金：辽宁省自然科学基金资助项目(20082079);辽宁省教育厅资助项目(L2010644;2009A767;2008822);沈阳市科技计划资助项目(F10-149-9-42)~~

摘　　要：背景:改变合金构成可以改善镁合金的耐腐蚀性和机械性能。目的:观察钙磷涂层对镁合金植入体周围软组织中骨形成蛋白2表达的影响。方法:将27只兔随机分成3组,分别在兔下颌骨内植入钙磷涂层镁合金螺钉、镁合金螺钉及钛合金,植入后1,2,3个月,采用反转录聚合酶链技术检测植入体周围软组织中骨形成蛋白2mRNA的表达情况。结果与结论:钙磷涂层镁合金螺钉组、镁合金螺钉组周围软组织中骨形成蛋白2mRNA的表达,随时间的增加逐渐增高,钙磷涂层镁合金螺钉组内各时间点间骨形成蛋白2mRNA的表达水平差异有显著性意义(P<0.05),且镁合金螺钉组植入后1,2个月周围软组织中骨形成蛋白2mRNA的表达水平高于钙磷涂层镁合金螺钉组(P<0.05)。显示钙磷涂层能够促进骨形成蛋白2mRNA的表达,并抑制其过度表达,有利于早期成骨过程,表现出良好的生物活性。BACKGROUND： The alloy composition adjustment can improve the corrosion resistance and mechanical properties of magnesium alloys. OBJECTIVE： To investigate bone morphogenetic protein-2 （BMP-2） expression in the soft tissues around calcium phosphate coating magnesium alloys. METHODS： Twenty-seven healthy adult male Japanese white rabbits were randomly divided into calcium phosphate coating group, untreated group and titanium alloy group. We implanted calcium phosphate coating and untreated AZ31B magnesium alloy screws into the rabbits＇ mandible, and titanium alloy group served as the control. The animals were sacrificed after 1, 2 and 3 months, and BMP-2 mRNA expressions were measured by reverse transcription-PCR. RESULTS AND CONCLUSION： With time passed, the BMP-2 mRNA expression showed a gradual increase for calcium phosphate coating group and untreated group, and significant differences exited in the calcium phosphate coating group at different time points （P 〈 0.05）. The BMP-2 mRNA expression of the untreated group was significantly higher than that of the coating group at 1 and 2 months after implantation （P 〈 0.05）. Calcium phosphate coating can promote the mRNA expression of BMP-2, which is beneficial to the early osteogenesis and shows good biological activity.

关 键 词：镁合金 涂层 骨形成蛋白2 磷酸三钙 螺钉 

分 类 号：R318[医药卫生—生物医学工程]