四种烤瓷冠基底金属浸提液干预人牙龈成纤维细胞尿纤溶酶原激活剂和Ⅰ型纤溶酶原激活物抑制剂的表达  

Effects of four kinds of base metal alloy crown extract on expression levels of urokinase-type plasminogen activator and plasminogen activator inhibitor-1 in human gingival fibroblasts

作　　者：芦琳[1] 黄克强[1] 胡静[1] 杜雅[1] 

机构地区：[1]辽宁医学院附属第二医院正畸科,辽宁省锦州市121004

出　　处：《中国组织工程研究》2012年第21期3915-3918,共4页Chinese Journal of Tissue Engineering Research

摘　　要：背景:修复材料的生物相容性是决定临床修复效果的重要因素之一。目的:通过比较4种金属浸提液对人牙龈成纤维细胞尿纤溶酶原激活剂和Ⅰ型纤溶酶原激活物抑制剂表达的差异探索其生物相容性。方法:选用活体牙龈,原代培养人牙龈成纤维细胞,采用镍铬、钴铬、纯钛及金钯合金4种金属浸提液进行干预,以未进行干预的细胞作为对照。结果与结论:ELISA检测结果显示镍铬和钴铬合金浸提液干预后细胞尿纤溶酶原激活剂表达增加;免疫荧光实验结合电镜观察发现镍铬和钴铬合金浸提液干预的细胞胞质荧光染色深,分布均匀,遍布整个细胞,提示细胞Ⅰ型纤溶酶原激活物抑制剂表达增加。而纯钛和金钯合金浸提液对细胞尿纤溶酶原激活剂和Ⅰ型纤溶酶原激活物抑制剂的表达无影响。说明纯钛和金钯合金的生物相容性优于镍铬和钴铬合金。BACKGROUND： Biocompatibility of repairing materials is an important factor for clinical effects. OBJECTIVE： To evaluate the material biocompatibility in oral repair by analyzing different expressional levels of urokinase-type plasminogen activator （uPA） and plasminogen activator inhibitor-1 （PAl-l） of human gingival fibroblasts （HGFs） in four types of metal extractions. METHODS： Healthy gums were obtained to cultivate HGFs. Levels of PAl-1 and uPA were examined by immunofluorescence and enzyme linked immunosorbent assay （ELISA） detection after different procedures： Co-Cr, Ni-Cr, Ti and rhotanium metal extractions. HGFs with no treatment served as controls. RESULTS AND CONCLUSION： ELISA results showed an increase in uPA levels after treatment with Co-Cr and Ni-Cr metal extractions. Immunofluorescence detection and electron microscope revealed deep-stained and even-distributed cytoplasm after treatment with Co-Cr and Ni-Cr, indicating PAl-1 levels were increased. There were changes in the levels of uPA and PAl-1 after treatment with Ti and gold alloy metal extractions. Biocompatibility of Ti and rhotanium is better than that of Co-Cr and Ni-Cr.

关 键 词：金属浸提液 牙龈成纤维细胞 尿纤溶酶原激活剂 Ⅰ型纤溶酶原激活物抑制剂 镍铬合金 钴铬合金 纯钛 金钯合金 

分 类 号：R318[医药卫生—生物医学工程]