大鼠骨髓源内皮祖细胞分化过程Notch信号通路活化及榄香烯干预机制的探讨  

Activation of Notch signal pathway in the process of differentiation of endothelial progenitor cells derived from rat bone marrow and intervention mechamism of β-elemene in it

作　　者：孟晓[1] 宋颖[1,2] 刘云鹏[3] 杨向红[1] 

机构地区：[1]中国医科大学附属盛京医院病理科,辽宁沈阳110004 [2]新乡医学院病理教研室,河南新乡453000 [3]中国医科大学附属第一医院肿瘤内科,辽宁沈阳110001

出　　处：《中华肿瘤防治杂志》2012年第7期489-493,共5页Chinese Journal of Cancer Prevention and Treatment

摘　　要：目的:观察大鼠骨髓来源内皮祖细胞分化过程中Notch信号通路的活化情况及β-榄香烯对该通路的影响。方法:大鼠骨髓来源内皮祖细胞(EPCs)的原代培养;RT-PCR法检测不同培养时间(0、4、7、10、14d)和不同浓度β-榄香烯(0、5、10、20μg/mL)干预的EPCs中CD133、vWF和Notch信号通路靶基因Hey-2和Hes-1的表达。结果:随着EPCs培养时间的延长,CD133表达明显减弱,vWF表达无显著变化,Notch信号通路靶基因Hey-2和Hes-1表达增加;随着β-榄香烯浓度的增加,Hey-2和Hes-1表达逐渐减弱。结论:在EPCs分化过程中,Notch信号通路发生活化;β-榄香烯对EPCs的分化和Notch信号通路的活化有明显的抑制作用,且具有浓度依赖性。推测,β-榄香烯可能会抑制EPCs分化为内皮细胞,抑制肿瘤血管生成,进而抑制肿瘤的生长和转移。OBJECTIVE：To observe the activation of Notch signal pathway in EPC which come from rat bone marrow and the effect of β-elemene for this pathway. METHODS： Endothelial progenitor cell from rat bone marrow culture, RT-PCR was used to detect CD133, vWF, and the target gene Hey-2 and Hes-1 of Notch pathway in EPC, which was cultured for different time （0,4,7,10,14 days） and interfered with different color of β-elemene（0,5,10,20 μg/mL） for 24 hours. RESULTS： With the extension of culture time, the expression of CD133 was down-regulation, vWF was not changed notable; the target gene Hey-2 and Hes-1 of Notch pathway were up-regulation; with the addition of color of β-elemene, the expression of Hey-2 and Hes-1 were down-regulation. CONCLUSIONS： In the process of EPC differentiate to endothelial cell, Notch pathway is activated; β-elemene can inhibit this activation, and the inhibition independented on the color of β-elemene. So we suppose that β-elemene may inhibit EPC differentiates to endothelial cell and tumor anglogenesis, thereby inhibit growth and metabasis of tumor.

关 键 词：内皮祖细胞 Notch信号通路 Β-榄香烯 分化 

分 类 号：R285.5[医药卫生—中药学]