黄芩苷对脊髓缺血神经保护作用的研究  

The Neuroprotective Effect of Baicalin on Spinal Cord Ischemic Injury

作　　者：石松生[1,2] 王立胜[1] 陈春美[2] 王春华 杨卫忠[2] 王锐[2] 

机构地区：[1]福建医科大学协和临床医学院,福州350001 [2]福建医科大学附属协和医院神经外科,福州350001 [3]福建省神经外科研究所,福州350001

出　　处：《福建医科大学学报》2012年第2期108-112,共5页Journal of Fujian Medical University

基　　金：福建省卫生厅创新课题(2009-CXB-17);福建省教育厅科技项目课题(JA05259)

摘　　要：目的探讨黄芩苷对脊髓缺血神经保护作用机制的研究。方法建立新西兰大白兔脊髓缺血模型,分为模型组、高剂量组、低剂量组及假手术组。采用H-E染色、原位末端转移酶标记技术(TUNEL)、免疫组织化学、逆转录反应系统(RT-PCR)等技术检测N-甲基-D-天门冬氨酸受体(NMDAR)、诱导型一氧化氮合酶(iN-OS)、半胱氨酸天冬氨酸蛋白酶3(Caspase-3)的表达水平,观察不同剂量黄芩苷对脊髓缺血的神经保护作用。结果模型组脊髓正常结构完全消失,低剂量组结构较完整,假手术组脊髓结构正常。高剂量组缺血损害情况介于低剂量组于假手术组之间。凋亡指数、表达NMDAR、iNOS、Caspase-3的阳性细胞百分比及相应mRNA表达水平:模型组最高,低剂量组、高剂量组、假手术组则依次降低,差别具有统计学意义(P<0.05)。结论黄芩苷能抑制神经细胞凋亡,对脊髓缺血具有神经保护作用。Objective To study the neuroprotective mechanism of baicalin on spinal cord ischemic injury.Methods Spinal cord ischemia models of New Zealand white rabbit were established.and then divided into model group,high-dose group,low-dose group and sham-operation group randomly.H-E staining,TUNEL assay,immunohischemistry and RT-PCR were employed to detect expression levels of NMDAR,iNOS,Caspase-3,and the neuroprotective effects of different doses of baicalin on spinal cord ischemic injury were studied.Results Normal structures of the spinal cord completely disappeared in the model group.The spinal cord structures from low-dose baicalin treated group improved compared to those from control group.Sham-operation group showed normal spinal cord structures.The ischemic damages in spinal cord from high dose baicalin treated group were less severe compared to those from low-dose group.Apoptotic index,percentage of NMDAR,iNOS,Caspase-3 positive cells and the corresponding mRNA levels from the model group,low-dose group,high-dose group and sham-operation group gradually decreased with statistical significance.Conclusion Baicalin has neuroprotective effect on inhibiting neuronal cell apoptosis induced by spinal cord ischemic injury.

关 键 词：黄芩苷 脊髓 缺血 创伤和损伤 受体 N-甲基-D-天冬氨酸 半胱氨酸天冬氨酸蛋白酶3 疾病模型 动物 

分 类 号：R285.5[医药卫生—中药学]