丹栀逍遥散防治多囊卵巢综合征模型大鼠的P450arom及ERK通路研究  

Effects of Dan Zhi Xiao-Yao Power on rat polycystic ovary syndrome and its regulation of P450arom and ERK 1/2

作　　者：刘颖[1,2] 邢福祺[1] 张丽华[3] 靖林林[3] 孙学刚[1] 

机构地区：[1]南方医科大学南方医院,广东广州510515 [2]广东省人民医院,广东广州510080 [3]南方医科大学中西医结合医院广东广州510515

出　　处：《暨南大学学报（自然科学与医学版）》2012年第2期144-147,共4页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：国家自然科学基金项目(81173168);广东省医学科学研究基金(A2010036)

摘　　要：目的:观察丹栀逍遥散(DZXYP)对多囊卵巢综合征(PCOS)模型大鼠的作用及机制。方法:用皮下埋植左旋18-甲基炔诺酮硅胶棒联合皮下注射人绒毛膜促性腺激素的方法诱导PCOS模型,化学发光法检测血清黄体酮(LH)、卵泡刺激素(FSH)、雌二醇(E2)和睾酮(T),免疫组化检测细胞色素P450芳香化酶(P450arom)的表达,western blotting检测P450arom及细胞外信号调节激酶(ERK)1/2的活化。结果:DZXYP降低LH(P<0.05)和T(P<0.01)水平,增高FSH水平(P<0.01);增加P450arom的表达,降低ERK 1/2磷酸化。结论:P450arom表达降低是大鼠该PCOS模型发病的因素之一,DZXYP可能通过抑制ERK 1/2磷酸化,增加P450arom表达防治PCOS。Aim：To investigate the effects of DanZhi XiaoYao Power（DZXYP） on rat polycystic ovary syndrome（PCOS） rats and its regulation on aromatase cytochrome P450（P450arom） and extracellular signal-regulated kinase ERK 1/2.Methods： Capsulae Levonorgestreli Silasticus and human chorionic gonadotrophin were used to induce PCOS model in rats.LH,FSH,E2 and T were measured by chemiluminescence.Immunohistochemistry was used to analyze the expression of P450arom and Western blotting was used to determine the expresson of P450arom and phosphorylation of ERK 1/2.Results： DZXYP reduced the level of LH,T and increased the level of FSH.It also increased the expression of P450arom and decreased the phosphorylation of ERK 1/2.Conclusion：The lowered expression of P450arom is an important factor in the pathogenesis of the PCOS model.DZXYP might ameliorate PCOS by increasing P450arom expression and reducing ERK 1/2 phosphorylation.

关 键 词：多囊卵巢综合征(PCOS) 丹栀逍遥散(DZXYP) 细胞色素P450芳香化酶 细胞外信号调节激酶1/2 

分 类 号：R271.1[医药卫生—中医妇科学]