疏肝健脾方药对非酒精性脂肪性肝炎大鼠肝组织TNF-α、IL-6及IL-1的影响  

Effects of soothing liver and invigorating spleen recipes of TCM on hepatic inflammatory cytokines of rats with nonalcoholic steatosishepatitis

作　　者：金玲[1] 杨钦河[2] 张玉佩[2] 王文晶[2] 韩莉[1] 陈友鹏[3] 冯高飞[2] 何秀敏[2] 赵亚凤[2] 沈灿宏[2] 杨晓蕾[2] 李光秋[2] 闫海震[2] 黄进[2] 

机构地区：[1]暨南大学附属第一医院中医科,广东广州510630 [2]暨南大学医学院中医系,广东广州510632 [3]暨南大学附属第一医院感染科,广东广州510630

出　　处：《暨南大学学报（自然科学与医学版）》2012年第2期161-166,共6页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：国家自然科学基金项目(30973694);国家大学生创新性实验计划项目(091055930)

摘　　要：目的:观察疏肝健脾方药对高脂饮食诱导的非酒精性脂肪性肝炎(NASH)大鼠肝组织肿瘤坏死因子(TNF-α)、白细胞介素6(IL-6)及白细胞介素1(IL-1)水平的影响,探讨其抗NASH的作用机制。方法:采用高脂饲料喂养复制NASH大鼠实验模型,同时各药物组分别灌服疏肝方(柴胡疏肝散)、健脾方(参苓白术散)和综合方(柴胡疏肝散和参苓白术散的合方)的不同剂量进行干预,16周后处死大鼠,用全自动生化分析仪检测血脂及肝脂的浓度;常规HE染色和油红O染色观察肝组织病理变化;用酶联免疫法(ELISA)检测肝组织匀浆中炎症细胞因子TNF-α、IL-6及IL-1的水平。结果:与正常组相比,模型组大鼠肝细胞脂肪变性明显,血清总胆固醇(TC)、低密度脂蛋白(LDL)和肝组织TC、甘油三酯(TG)及肝组织中炎症因子TNF-α、IL-6、IL-1的浓度显著升高(P<0.01);各药物组大鼠肝组织TNF-α、IL-6及IL-1的水平与模型组相比有均有不同程度下降,以综合方高剂量组和健脾方高剂量组下降最为明显(P<0.01、P<0.05),脂质检测以综合方高剂量组的血清TG及肝组织TC、TG下降最为明显(P<0.01)。结论:疏肝健脾方药有抗大鼠NASH的作用,其机制可能与其抑制肝组织炎症因子TNF-α、IL-6及IL-1的表达及降低脂质浓度有关。Aim：To investigate the effects of soothing liver and invigorating spleen recipes on the levels of inflammatory cytokines in liver tissue of rats with nonalcoholic steatosishepatitis（NASH）,and to explore the mechanisms of the anti-NASH effect of the drugs.Methods： The models of NASH rats were established with high fat diet,then the rats were fed with soothing liver recipes,invigorating spleen recipes and integrated recipes respectively in diferent groups.The rats were sacrificed after 16 weeks＇ medication,blood and liver samples were collected for TC and TG test,pathomorphology analysis,and TNF-α,IL-1 and IL-6 determination.Results： In these model groups,the level of TC and TG in the serum and liver tissue,the level of TNF-α 、IL-1 and IL-6 in liver tissue in the model group were increased significantly（P0.01）,and the fatty degeneration in hepatocytes in model group were more obvious.In these model groups,the levels of TNF-α 、IL-1 and IL-6 in liver tissue were decreased significantly in the high-dose invigorating spleen group and the high-dose integrated group（P0.05、P0.01）,the level of TG in serum and TC、TG in liver tissue were decreased significantly in the high-dose integrated group（P0.01）.Conclusion：The Soothing liver and invigorating spleen recipes have certain therapeutic effects on rats with NASH,the mechanisms may be their decreasing blood-lipoids、liver lipids levels and TNF-α,IL-1 and IL-6 levels in liver tissue.

关 键 词：非酒精性脂肪性肝炎 疏肝健脾方药 炎症因子 大鼠 

分 类 号：R285.5[医药卫生—中药学]