甘草对红花中羟基红花黄色素A药代动力学的影响  

Effects of Radix Glycyrrhizae on the Pharmacokinetics of Hydroxysafflor Yellow A in Carthamus Tinctorius L.

作　　者：骆晶[1] 王晓雯[2] 杨凌鉴[3] 陈向梅[3] 王世祥[3] 彭宁[2] 刘勤社[2,3] 郑晓晖[3,4] 李联社[1] 

机构地区：[1]陕西中医学院,陕西咸阳712046 [2]陕西省人民医院,陕西西安710068 [3]西北大学生命科学学院,陕西西安710069 [4]清华大学深圳研究院,广东深圳518057

出　　处：《中国药业》2012年第9期12-14,共3页China Pharmaceuticals

基　　金：国家科技支撑计划课题;项目编号:2008BAI51B01;陕西省"13115"科技创新工程重大科技专项;项目编号:2009ZDKG-85;陕西省中医药管理局项目;项目编号:1c19;深圳市科技工贸和信息化委员会项目;项目编号:CXB201005260069A

摘　　要：目的选择代表复方关键配伍关系的红花-甘草(君-使)药对为研究对象,通过探讨甘草对红花水提液中有效成分羟基红花黄色素A药代动力学的影响,揭示复方中使药调和作用的物质基础及其机制。方法以三氯乙酸沉淀法去除血浆蛋白,反相高效液相色谱外标法测定羟基红花黄色素A的含量。以DAS 2.0软件拟合药时曲线,计算药代动力学参数。结果配伍前后,羟基红花黄色素的药代动力学曲线分别符合二室开放模型和一室开放模型;配伍后,羟基红花黄色素A的吸收半衰期和消除半衰期明显缩短,药时曲线下面积明显减小。结论甘草促进红花的吸收和消除为其发挥调和作用的机制之一。Objective Flos Carthami and Radix Glycyrrhizae were selected as exam ple representing key synergistic relationship of complex prescription to elucida te the harmonizing effect and substantial foundation of Radix Glycyrrhizae in a formula by investigating the effect of Radix Glycyrrhizae on pharmacokinetic of hydroxysafflor yellow A,an active ingredient in Flos Carthami.Methods Trichloro acetic acid was used to remove plasma protein in the samples.Reverse phase high performance liquid chromatography with external standard method was employed to determine the concentration of hydroxysafflor yellow A.Software of DAS 2.0 was e mployed to regress the drug-plasma concentration curve and calculate pharmacoki netic parameters of hydroxysafflor yellow A.Results The pharmacokinetic profiles of hydroxysafflor yellow A confirm to one and two compartment open model for gr oups of Radix Glycyrrhizae and Carthamus tinctorius L.coupled with Radix Glycyrr hizae,respectively.The absorption half-life and elimination half-life of HSYA shortened significantly after compatibility.Meanwhile,the area under the curve（AUC） was significantly decreased.Conclusion Radix Glycyrrhizae exerts its harmo nizing effect by enhancing absorption and elimination of hydroxysafflor yellow A.

关 键 词：高效液相色谱法 药代动力学 红花 甘草 羟基红花黄色素A 

分 类 号：R282.71[医药卫生—中药学] R284.1[医药卫生—中医学]