BMP-2和p38MAPK在小鼠骨髓间充质干细胞向成骨细胞分化中的作用  

Effect of BMP-2 and p38MAPK on differentiation of mouse BMSCs to osteoblasts

作　　者：孙欣[1] 曾荣[1] 郭伟韬[1] 肖启贤[1] 王斌[1] 黄云[1] 林颢[1] 

机构地区：[1]广东医学院附属医院骨科,广东湛江524001

出　　处：《广东医学院学报》2012年第1期1-3,12,共4页Journal of Guangdong Medical College

基　　金：广东医学院附属医院青年科研基金(No.2009k10);广东医学院青年基金(No.XQ0846);湛江市财政资金科技专项竞争性分配项目([2009]163号);湛江市科技攻关计划项目(湛科[2011]97号)

摘　　要：目的观察骨成型蛋白(BMP)-2和p38丝裂原激活蛋白激酶(MAPK)在小鼠骨髓间充质干细胞(BMSC)向成骨细胞分化中的作用。方法体外分离和扩增小鼠BMSC,分为对照组、BMP-2组及阻断剂组,其中BMP-2组加入BMP-2,阻断剂组加入SB203580(p38MAPK通路阻断剂)和BMP-2。测定碱性磷酸酶(ALP)活性、钙沉积量、p38MAPK表达。结果与对照组相比,BMP-2组BMSC中ALP活性和钙沉积量增加(P<0.01),p38MAPK表达较早出现。阻断剂组p38MAPK表达明显延迟,与对照组相似。结论 BMP-2可通过p38MAPK通路促进BMSC向成骨细胞分化。Objective To observe the effect of bone morphogenetic protein（BMP）-2 and p38MAPK on the differentiation of mouse bone-marrow-derived mesenchymal stem cells（BMSCs） to osteoblasts.Methods In vitro isolated and amplified Mouse BMSCs were divided into control,BMP-2 and antagonist groups,and the latter two groups were added by BMP-2 and SB203580（p38MAPK pathway antagonist） and BMP-2,respectively.Alkaline phosphatase（ALP） activity,calcium deposition and p38MAPK expression were measured.Results Compared with control group,ALP activity and calcium deposition of BMSCs increased（P0.01）,and p38MAPK expression occurred early in BMP-2 group.Time course of p38MAPK expression was delayed in antagonist group,similar to that in control group.Conclusion BMP-2 may induce osteoblastic diferentiation in mouse BMSCs via p38MAPK pathway.

关 键 词：骨成型蛋白2 P38MAPK 骨髓间充质干细胞 成骨细胞 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]