基于MSP430单片机的便携式电化学DNA检测系统  

Portable Electrochemical DNA Detection System Based on MSP430

作　　者：李远[1,2] 蒋稼欢[2] 王静[2] SUNG K.L.Paul 

机构地区：[1]重庆医科大学附属永川医院中心实验室,重庆402160 [2]重庆大学生物工程学院生物流变科学与技术教育部重点实验室,重庆400030 [3]美国加州福利亚大学圣地亚哥分校生物工程与整形外科系

出　　处：《仪表技术与传感器》2012年第4期52-54,63,共4页Instrument Technique and Sensor

基　　金：国家自然科学基金项目(30870607);重庆市自然科学基金项目(2008BB5192)

摘　　要：为实现现场DNA杂交检测,设计了一套便携式电化学DNA检测系统;系统以MSP430单片机为主控芯片,结合外围恒电位电路和控制软件,实现电化学循环伏安法检测;电化学DNA生物传感器利用原位电化学共聚合技术将DNA探针包埋固定在导电聚吡咯分子网络中,利用DNA杂交反应改变聚吡咯氧化还原过程中的离子交换动力学行为的原理实现DNA杂交检测;设计的DNA检测系统硬件结构检测,低功耗,精确实现电化学循环伏安法检测;配合电化学DNA生物传感器,可实现无标记DNA检测,检测下限达到5×10-18M,适合在野和个性化DNA检测。This paper designed a portable electrochemical DNA detection system for DNA hybridization detection in field.The electrochemical detection system,which used MSP430 singlechip as the main microcontroller,could implement electrochemical cyclic voltammetry by combining with peripheral potentiostat circuits and control software.The DNA biosensor was prepared by entrapment DNA probe into polypyrrole molecule network by electrochemical in-situ co-polymerization,DNA hybridization detection was realized by monitoring the ion-exchange behavior of polypyrrole during redox process.This constructed DNA detection system has the merits of simple structure and low power-consume and the detection limit was as low as 5 × 10-18M.This system was suit for in-field and point-of-care DNA detection.

关 键 词：MSP430 DNA传感器 恒电位仪 聚吡咯 便携式 

分 类 号：TQ035[化学工程]