白藜芦醇改善糖尿病血管炎症反应及其与RAGE信号的相关性  

Resveratrol protecting the aorta against inflammation reaction associated with RAGE signal in diabetic rats

作　　者：刘涛[1] 巩增锋[2] 尹浩 景玉宏[3] 

机构地区：[1]兰州大学第一医院东岗院区药械科,730000 [2]兰州大学第一医院东岗院区内科,730000 [3]兰州大学基础医学院人体解剖与组织胚胎学研究所

出　　处：《中国糖尿病杂志》2012年第6期456-460,共5页Chinese Journal of Diabetes

基　　金：国家自然科学基金(30872731);甘肃省自然基金

摘　　要：目的在糖尿病动物模型上研究白藜芦醇(RSV)对糖尿病血管损伤的保护效应及可能机制。方法 STZ单次低剂量(40mg/kg)经股静脉注射,联合高脂饮食,建立糖尿病模型。利用酶底物分析技术检测血液中糖化血清蛋白的含量,在整体主动脉组织上利用Oil Red检测血管内壁脂肪斑块沉积。利用半定量RT-PCR技术检测主动脉组织中TNF-α及IL-6的水平。利用Western blotting技术检测主动脉组织中RAGE表达水平。结果 RSV持续给药可以减轻糖尿病大鼠血液中糖化血清蛋白的含量,抑制主动脉组织中TNF-α及IL-6的水平,同时抑制RAGE的高表达。RSV也显著减少了糖尿病所致的主动脉内壁脂肪斑块沉积。结论 RSV可以保护糖尿病导致的血管损伤,这一作用可能和RSV减轻糖基化产物聚集,抑制RAGE信号,减轻血管炎症反应,减少血管壁脂肪斑块沉积有关。Objective To study the protective effect and underlying mechanism of resveratrol treatment on vasculature injury in diabetic rats. Methods Diabetic animal models were established by single injection of STZ（40 mg/kg） through femoral vein together with high fat diet. Blood fructosamine was measured by using reaction of enzyme-substrate for four months. Lipid plaques were tested by using Oil red staining in intact aorta. Levels of TNF-α and IL-6 in aorta tissue were analyzed by using semiquantitative RT-PCR technique. Also, expression of RAGE in aorta was tested by Western blot method. Results Resveratrol treatment attenuated the amount of blood fructosamine, suppressed the expression of TNF-α and IL-6, and decreased the expression of RAGE in aorta as well as ameliorated the deposition of lipid plaques in inner wall of aorta in diabetic rats. Conelmion Resveratrol treatment can protect the vasculature injured by diabetes. This protective effect may relate with its functions in attenuation of accumulation of advanced glycation end product, inhibition of the RAGE signal, amelioration of the vasculature inflammatory reaction, and reduction of deposition of lipid plaques in diabetic rats.

关 键 词：白藜芦醇 糖尿病 糖基化产物及其受体 血管 

分 类 号：R285.5[医药卫生—中药学]