ELISPOT及ELISA鉴别HBV感染免疫状态的研究  

Study on immune status of patients with HBV infection identified by ELISPOT and ELISA

作　　者：施理[1] 许小珍[1] 符健[1] 林锋[1] 贾杰[1] 陈所贤[1] 蔡笃运[1] 王英[1] 

机构地区：[1]海南省人民医院传染科,海南海口570311

出　　处：《中华医院感染学杂志》2012年第11期2265-2267,共3页Chinese Journal of Nosocomiology

基　　金：海南省自然基金(200831);海南省卫生厅项目(200816)

摘　　要：目的比较酶联免疫斑点法(ELISPOT)、酶联免疫吸附试验(ELISA)鉴别乙型肝炎病毒(HBV)感染免疫状态。方法临床诊断的慢性HBV感染者免疫清除期、免疫耐受期患者各16例,常规分离外周血单个核细胞(PBMC),体外用HBcAg或培养液刺激,分别用ELISPOT及ELISA方法,检测两组患者PBMC分泌的r-干扰素(INFr)。结果用ELISA方法,两组患者PBMC受HBcAg刺激分泌IFNr,分别为(655.25士3.80)、(578.37±7.73)ng/L,阳性率分别为68.81%、12.50%,差异有统计学意义(P<0.01);用ELISPOT方法,两组患者PBMC受HBcAg刺激分泌IFNr,阳性率分别为87.50%、0,二者差异有统计学意义(P<0.01)。结论免疫清除期、免疫耐受期HBV感染者,PBMC对HBcAg刺激的免疫反应不同,鉴别二者,ELISPOT优于ELISA。OBJECTIVE To compare the immune status of patients with HBV infection by enzyme link immune spot（ELISPOT）and enzyme-linked immunosorbent assay（ELISA）.METHODS A total of 32 patients with chronic HBV infection were selected,16 patients in clearance period and 16 patients in tolerance period were included.The peripheral blood mononuclear cells（PBMC） from the patients were isolated by routine method,which were stimulated with HHBcAg in vitro or media in vitro.The interferon-r（IFNr） produced by PBMC were measured by the method of ELISPOT and ELISA.RESULTS By the means of ELISA,the IFNr produced by PBMC under the stimulation of HBcAg of the two groups of patients were（655.25士3.80 ng/L and（578.37±7.73） ng/L,the positive rates were 68.81% and 12.50%,respectively,the difference was statistically significant（P〈0.01）;by the means of ELISPOT method,the positive rates of IFNr produced by PBMC under the stimulation of HBcAg were 87.50% and 0,respectively,the difference was statistically significant（P〈0.01）.CONCLUSION For the patients with HBV infections during immune serum clearance period and immune tolerance period,PBMC has different response to the immune status under the stimulation of HBcAg,ELISPOT is superior to ELISA in differentiating the two.

关 键 词：酶联免疫斑点法 酶联免疫吸附试验 乙型肝炎病毒感染者 R-干扰素 

分 类 号：R512.62[医药卫生—内科学]