联合检测幽门螺杆菌CagA、VacA、Ure、Hsp60及RdxA的临床价值  

Clinical value of combined detection of CagA,VacA,Ure,Hsp60 and RdxA in Helicobacter pylori

作　　者：陈海潮 单平囡 许德顺 

机构地区：[1]浙江省绍兴县中心医院 [2]中国医大绍兴医院检验科,浙江绍兴县312030

出　　处：《中华医院感染学杂志》2012年第11期2268-2270,共3页Chinese Journal of Nosocomiology

摘　　要：目的探讨联合检测幽门螺杆菌细胞毒素相关蛋白(CagA)、空泡毒素相关蛋白(VacA)、尿素酶(Ure)、热休克蛋白60(Hsp60)和氮素还原酶(RdxA)在胃、十二指肠疾病中的临床价值。方法对486例患者以免疫斑点试验(蛋白芯片)检测幽门螺杆菌CagA、VacA、Ure、Hsp60及RdxA抗体,对照组106例为健康体检人员。结果患者组中CagA、VacA、Ure、Hsp60的总阳性率分别为65.02%、52.67%、71.40%、11.74%,均明显高于对照组(P<0.01);CagA、VacA和Ure检测对萎缩性胃炎、胃癌的阳性率为80.00%～90.00%,对胃、十二指肠溃疡的阳性率为70.00%～78.00%,其余的阳性率均<74.00%,但VacA、Ure、Hsp60检测对胃癌的阳性率均为89.47%。结论 5种Hp抗体检测对表浅性胃炎、萎缩性胃炎、胃、十二指肠溃疡及胃癌诊治有较高参考价值。OBJECTIVE To study the clinical value of combined detection of CagA,VacA,Ure,Hsp60,and RdxA in Helicobacter pylori in the diagnosis and treatment of the gaster-duodenum disease.METHODS The antibodies of CagA,VacA,Ure,Hsp60 and RdxA of H.pylori for 486 patients and 106 personnel of health-examination were detected by the immunospot test（protein array）.RESULTS The total positive rates of CagA,VacA,Ure,Hsp60 and RdxA（65.02%,52.67%,71.40%,11.73%,respectively） in the patient group were significantly higher than those in the control group（P〈0.001）;the positive rates of CagA,VacA,and Ure for the detection of atrophic gastritis and gastric cancer varied from 80.00% to 90.00%,gaster-duodenum ulcer varied from 70.00% to 78.00%,others less than 74.00%,but the positive rates of VacA,Ure and Hsp60 for the detection of gastric cancer were 89.47% all.CONCLUSION The detection of 5 Hp antibodies has significant value in diagnosis and treatment of superficial gastritis,atrophic gastritis,gaster-duodenum ulcer,and gastric cancer.

关 键 词：幽门螺杆菌 抗体检测 免疫斑点试验 胃、十二指肠疾病 

分 类 号：R378[医药卫生—病原生物学]