内毒素对肝脏p53表达及Ca^(2+)浓度的影响  

Effect of endotoxin on expression of p53 and concentration of Ca^(2+) in liver of rats

作　　者：李勇[1,2] 高洪[1] 严玉霖[1] 杨桂梅[3] 孔令青[1] 刘海峰[1] 彭洁[4] 

机构地区：[1]云南农业大学动物科学技术学院,云南昆明650201 [2]云南省药物研究所GLP中心,云南昆明650111 [3]昆明医学院,云南昆明650031 [4]云南农业职业技术学院,云南昆明650212

出　　处：《中国兽医学报》2012年第6期881-883,888,共4页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30560113);云南省高端科技人才引进计划项目(2009CI125);云南省现代农业生猪产业技术体系建设项目(云财农[2009]171号)

摘　　要：为探讨内毒素在致大鼠肝损伤过程中对肝脏p53表达及Ca2+浓度的影响,将48只SD大鼠随机分2组:Ⅰ组为NS对照组;Ⅱ组为ET致病组,2组大鼠分别于第3、4、8、12h每组各处死6只,采集肝脏分别用于p53表达的Western-blotting检测和Ca2+浓度的FCM检测。结果显示,Ⅱ组p53的表达明显高于Ⅰ组(P<0.01),且Ⅱ组一直呈上升趋势;Ⅱ组Ca2+的荧光指数明显高于Ⅰ组(P<0.01),且一直呈上升趋势。结果表明,ET能有效上调肝细胞p53蛋白的表达及Ca2+浓度。To investigate the effect of endotoxin on expression of p53 and concentration of Ca2＋ in the damaged liver of rats induced by ET. Forty-eight Sprague-Dawley rats were randomly divided into two groups., the normal saline controls group（group Ⅰ）and ET treatment group（group Ⅱ）. at 3,4,8 and 12 h past-treatment liver samples were taken for testing the expression of p53 by Western-blotting and the concentration of Ca^2＋ by FCM. For the expres- sion of p53 and the fluorescence index （FI） of Ca^2＋ ,groupⅡ was markedly higher than those of group Ⅰ （P〈0.01） with a rising trend. The results indicated that ET could up-regulate the p53 protein expression and the concentration of Ca^2＋ of hepatocyte in rats.

关 键 词：内毒素 肝脏 P53蛋白 Ca2+浓度 

分 类 号：S852.44[农业科学—基础兽医学]