Cd^(2+)与Zn^(2+)的联合效应对马氏珠母贝(Pinctada martensii)受精率的影响  

COMBINED EFFECTS OF Cd^(2+) AND Zn^(2+) ON THE FERTILIZATION RATE IN PINCTADA MARTENSII(DUNKER)

作　　者：邓云翠[1] 王辉[1] 李艳红[1] 朱晓闻[1] 罗明明[1] 王亚男[1] 

机构地区：[1]广东海洋大学水产学院,湛江524025

出　　处：《海洋与湖沼》2012年第2期323-328,共6页Oceanologia Et Limnologia Sinica

基　　金：广东省教育厅工程中心建设项目;GCZX-A0909号;广东省科技厅科技计划重大专项;2010B020201014号;科技部国家星火计划项目;2011GA780001号

摘　　要：采用中心组合设计和响应曲面法在实验室条件下研究了Cd2+与Zn2+对马氏珠母贝受精率的影响。Cd2+、Zn2+的浓度范围分别为0.030—20mg/L、0.100—20mg/L。结果表明:Cd2+与Zn2+两因子对马氏珠母贝受精率的一次效应极显著(P<0.01);Cd2+与Zn2+两因子一次互作效应极显著(P<0.01)。经响应曲面法分析,随着Cd2+与Zn2+浓度的增加受精率呈下降趋势,当Zn2+浓度在最低浓度(0.100mg/L)时,受精率随着Cd2+浓度的上升而下降;当Cd2+浓度在最低浓度(0.030mg/L)时,受精率随Zn2+浓度的上升而先下降后升高。本实验建立了马氏珠母贝受精率与Cd2+、Zn2+间关系的模型方程(R2=0.996,Adj.R2=0.987,Pred.R2=0.887,P<0.01)并可用于预测Cd2+、Zn2+对马氏珠母贝受精率的影响。Using the central composite face-centered design （CCF） and response surface methodology, the combined effects of Cdz＋ （0.030--20.000mg/L） and Zn2＋ （0.100--20.000mg/L） on the fertilization rate in Pinctada martensii were studied under laboratory conditions. The results showed that the effects of two factors Cd2＋ and Zn2＋ on the fertilization rate in P. martensii were highly significant （P〈0.01）, and effect of Cd2＋ was more significant than that of Zn2＋. The interaction between the two factors was also highly significant （P〈0.01）. Based on the response surface plots, it was found that with the increased concentration of Cd2＋ and Zn2＋ , the fertilization rate was decreased. When Zn2＋ concentration was at its lowest level （0.100mg/L）, the fertilization rate decreased with the increased Cd2＋ concentration. When Cd2＋ concentration was at its lowest level （0.030mg/L）, the fertilization rate was first decreased and then increased as the concentration of Zn2＋ increased. The model equation of the fertilization rate towards Cd2＋ and Zn2＋ was established, with R2 = 0.996, Adj.RE = 0.987 and Pred.R2 = 0.887 （P〈0.01）. The model equation could be practically used for forecasting the effects of Cd2＋ and Zn2＋ on the fertilization rate in P. martensii.

关 键 词：马氏珠母贝 受精率 Cd2+ Zn2+ 

分 类 号：Q789[生物学—分子生物学]