静水压力诱导牙鲆(Paralichthys olivaceus)四倍体的条件优化  

PRODUCTION OF VIABLE TETRAPLOID OLIVE FLOUNDER(PARALICHTHYS OLIVACEUS) BY HYDROSTATIC PRESSURE SHOCK

作　　者：衣启麟[1] 于海洋[1] 王兴莲[1] 王志刚[1] 王旭波[1] 齐洁[1] 张全启[1] 

机构地区：[1]中国海洋大学海洋生命学院海洋生物遗传育种教育部重点实验室,青岛266003

出　　处：《海洋与湖沼》2012年第2期382-388,共7页Oceanologia Et Limnologia Sinica

基　　金：山东省良种工程重大课题"优质抗病速生鱼类良种选育";2008-2014;国家863计划课题"海洋动物细胞分子育种关键技术";SS2012AA100802号;山东省海洋生物学泰山学者岗位经费资助;ts2010021号

摘　　要：采用静水压力抑制受精卵第一次卵裂的方法,进行了诱导牙鲆四倍体的研究。结果表明,将受精卵保持在(15.5±0.5)℃,从受精后70min开始用55MPa的压力处理6min后,正常仔鱼孵化率最高达到15.6%,此时的四倍体诱导率也最高达到63.3%。利用获得的最佳诱导条件处理获得了数千尾体长8—15cm的幼鱼,流式细胞仪检测和红血球长径测量结果显示,处理组幼鱼中四倍体约占13.3%,说明四倍体培育成功。该成果为开展牙鲆多倍体育种奠定了基础。Viable tetraploid olive flounder （Paralichthys olivaceus） were hatched under hydrostatic pressure shock. The result showed that optimal hatching rate for tetraploid individuals was achieved by 6 min of pressure shock （55Mpa） at （15.5±0.5）℃, 70min after fertilization. Under these conditions, the hatching rate of the treated eggs was 15.6%. Chromo- some observation and counting of maximum number of nucleolus showed that the tetraploid rate was 63.3%. Several thousands of young fish with body length of 8--15cm were obtained using this method. Both flow-cytometry and erythro- cyte diameter proved that 13.3% of the young fish were tetraploids. The successful production of tetraploid individuals provided possibilities for future polyploid breeding using this fish species.

关 键 词：牙鲆 四倍体 静水压力 多倍体育种 

分 类 号：Q953[生物学—动物学]