L-苯丙氨酸对小鼠妊娠早期胚胎发育及子宫局部肥大细胞的影响  

The influence of L-phenylalanine on the embryo development and uterus mast cells in early pregnant mice

作　　者：董玉兰[1] 白永平[1,2] 王子旭[1] 陈耀星[1] 李素琪[1] 韩亚楠[1] 刘冠慧[1] 

机构地区：[1]中国农业大学动物医学院,北京海淀100193 [2]中国农业科学院北京畜牧兽医研究所,北京海淀100193

出　　处：《中国兽医杂志》2012年第5期6-9,I0001,共5页Chinese Journal of Veterinary Medicine

基　　金：教育部博士点新教师基金(200800191004);国家大学生创新训练计划(101001919);国家自然科学基金(30700575)

摘　　要：为了研究交感神经对小鼠胚胎着床和发育的作用机制,本文筛选出合适的L-苯丙氨酸浓度(320mg/kg体重),处理孕鼠,观察胚胎着床和发育,通过甲苯胺蓝染色检测子宫局部肥大细胞的变化。研究结果显示,320mg/kg体重L-苯丙氨酸能显著降低胚胎着床数目,分别下降了45%(E7)和58%(E9),并且显著延缓胚胎的发育;与对照组相比,着床后子宫局部肥大细胞数目显著降低,分别在E5～E9降低了36.62%、64.59%和23.30%,差异显著。由本研究结果可以推断一定浓度的L-苯丙氨酸可以激活交感神经,影响妊娠早期子宫局部肥大细胞数量,从而改变子宫局部微环境,影响胚胎着床和发育。The purpose of this study is to investigate the role sympathetic nerve on the embryo implantation.The pregnant mice were treated by a suitable concentration of L-phenylalanine（320 mg/kg） and tested the number of mast cells by toluidine blue staining.The results showed that the number of embryo implantation decreased significantly by 45%（E7） and 58%（E9）,and delayed the development of embryos in the treated mice;The number of mast cells decreased by 36.62%、64.59% and 23.30% at E5, E7 and E9.The results suggest that L-phenylalanine with suitable concentration might activate the sympathetic nerve,and change the number of mast cells,which induces abnormal the uterus local microenviroment and play a harmful role in the implantation and development of embryo.

关 键 词：L-苯丙氨酸 胚胎着床 肥大细胞 

分 类 号：S852.2[农业科学—基础兽医学]