表没食子儿茶素没食子酸酯用于小鼠卵母细胞体外培养的效果观察  

EGCG effect on in-vitro culture of mouse oocytes

作　　者：李拥军[1] 李太坤[1] 李文婷[1] 张光景[1] 谷亚轻[1] 尚利青[1] 邹恒[1] 

机构地区：[1]扬州大学动物科学与技术学院 江苏省动物遗传繁育与分子设计重点实验室,江苏扬州225009

出　　处：《中国兽医杂志》2012年第5期17-19,共3页Chinese Journal of Veterinary Medicine

基　　金：江苏高校优势学科建设工程项目;扬州大学高层次人才科研启动基金项目(028889-5013500)

摘　　要：本试验以小鼠为动物模型,采用HTF和TCM-199两个基础培养体系,通过添加不同浓度的表没食子儿茶素没食子酸酯(EGCG),研究其对小鼠卵母细胞体外成熟、体外受精及其后期胚胎发育的影响。结果表明,在HTF和TCM-199体系中,培养卵丘-卵母细胞复合物(COCs)时EGCG的最佳添加量均为20μmol/L,可以显著提高卵母细胞的成熟率、受精率和胚胎发育率。过量添加EGCG则有负面的效应。综合比较,HTF体系的培养效果优于TCM-199体系。EGCG is a natural antioxidant extracted from tea.In this study,HTF and TCM-199 were used as foundation culture systems.By adding different concentration of EGCG,EGCG effect on IVM,IVF,and embryo development of mouse oocytes was investigated.The results indicated that 20 μmol/L EGCG had the best IVC effect on maturation rate,fertility rate,and embryonic development rate both in HTF and TCM-199.Overdose of EGCG could significantly degrade IVC effect.HTF culture system was better than TCM-199.

关 键 词：EGCG 卵母细胞 体外成熟 体外受精 胚胎发育 

分 类 号：S852.1[农业科学—基础兽医学]