卤酚化合物LM49对大鼠肝微粒体细胞色素P450的影响  

Effects of halophenol LM49 on microsomal CYP450 in Rats

作　　者：白玉[1] 张丽锋[1] 冯秀娥[1] 梁泰刚[1] 李青山[1] 

机构地区：[1]山西医科大学药学院,太原030001

出　　处：《中国药物与临床》2012年第5期574-576,共3页Chinese Remedies & Clinics

基　　金：国家"重大新药创制"科技重大专项(2009ZX09302-003);山西省归国留学人员基金(2008-51);北京大学天然药物与仿生药物国家重点实验室资助项目(20080210)

摘　　要：目的研究LM49对大鼠肝微粒体蛋白及细胞色素P450含量的影响。方法将大鼠分成空白对照组、溶剂对照组、阳性对照组(苯巴比妥组、地塞米松组、β-萘黄酮组)、LM49低、中、高剂量组。给药后采用超速离心法制备大鼠肝微粒体;BCA法测定大鼠肝微粒体蛋白浓度;Omura and Sato法测定大鼠肝微粒体细胞色素P450的含量。结果 给予不同剂量的LM49后,大鼠肝微粒体的蛋白及细胞色素P450含量均明显降低。低、中、高剂量组与对照组比较差异有统计学意义(P<0.05)。结论 LM49对大鼠肝微粒体细胞色素P450具有一定的抑制作用,可能引起肝药酶对某些药物代谢的改变。Objective To determine the effects of LM49 on microsomal protein and CYP450 in rats. Methods Rats were assigned into blank control group, solvent control group, positive control groups （phenobarbital, dexametha - sone and β-nephthoflavone） and LM49-treated （low, medium and high dosage） groups, respectively. Hepatic micro- somes were prepared using ultracentrifuge after administration of interventions. The concentration of microsomal pro - teins was determined using BAC approach, and the content of microsomal CYP450 was assayed via Omura and Sato method. Results Marked reduction in the concentration of microsomal proteins and CYP450 was associated with administration of LM49 with various （low, medium and high） dosages as compared with control group （all P 0.05）. Conclusion LM49 inhibits hepatic microsomal CYP450 and may result in changes of metabolism of certain medicines induced by liver drug enzymes in rats.

关 键 词：微粒体 肝 细胞色素P450酶系统 大鼠 

分 类 号：R285.5[医药卫生—中药学]