ATP生物荧光检测技术相关性基础研究  

Association study on adenosine triphosphate biofluorescence detection technology

作　　者：易滨[1] 刘军[1] 王芳[1] 涂显春[1] 赵晓晓[1] 赵江丽[1] 

机构地区：[1]中国人民解放军第309医院,北京100091

出　　处：《中国感染控制杂志》2012年第2期81-85,共5页Chinese Journal of Infection Control

摘　　要：目的测试三磷酸腺苷(ATP)生物荧光检测技术与菌落数和血液含量的关系。方法将大肠埃希菌ATCC8099菌液和健康人的血液分别稀释为10-1、10-2、10-3、10-4、10-5、10-6、10-7,加入裂解液、酶、ATP标准品,用荧光光度计测定2次相对发光值(RLU),按照公式(ATP含量=A1A2-A1×106)换算成ATP含量(amol);用血细胞分析仪测定血红蛋白值,以反映蛋白残留量。将各稀释度菌液、血红蛋白含量与ATP含量之间的关系进行曲线标定,判定其相关性。结果菌数对数值与ATP含量对数值(y=1.07x-0.55,R2=0.99);菌数对数值和ATPRLU对数值(y=1.14x+0.33,R2=0.99),都呈线性关系。不同RLU值推算的菌数理论值与实际值相比,差异无统计学意义。稀释后血红蛋白对数值与ATP RLU对数值(y=1.03x-8.42,R2=0.99)亦呈线性关系。结论 ATP生物荧光检测技术可通过ATP值和RLU来判断细菌和蛋白含量,从而判断医疗器械和物体表面的污染程度以及清洁、清洗效果,是一种较好、较敏感的快速检测新方法。Objective To test the relation between adenosine triphosphate（ATP） biofluorescence detection technology and bacterial colony forming unit（CFU） and blood content.Methods Escherichia coli（E.coli） ATCC 8099 and heathy controls＇ blood were diluted to the concentration of 10-1,10-2,10-3,10-4,10-5,10-6 and 10-7,respectively,then lysate,luciferase and ATP standard substance were added,relative light unit（RLU） values were determined twice by fluorimeter,ATP contents（amol） were converted according to formula（ATP content=A1 A2-A1×106）;hemoglobin values were measured by hematology analyzer to reflect protein residue level.Curve demarcate standardization was made to show the relation between diluted bacteria,hemoglobin content and ATP content.Results Bacterial CFU and ATP content logarithm values（y=1.07x-0.55,R2=0.99）,bacterial CFU and ATP RLU logarithm values（y=1.14x＋0.33,R2=0.99） showed linear relationship respectively.There was no significant difference between the logical values figured out by different RLU values and the actual values.After hemodilution,hemoglobin and ATP RLU logarithm values also showed linear relationship（y=1.03x-8.42,R2=0.99）.Conclusion ATP biofluorescence detection technology can detect the content of bacteria and protein through ATP value and RLU,it can determine contamination degree and clean effect of medical equipments and object surface,it＇s a new,sensitive and rapid detection method.

关 键 词：ATP 生物荧光 ATP生物荧光检测 清洗 感染控制 

分 类 号：R197.39[医药卫生—卫生事业管理]