益气解毒方对癌前病变鼻咽黏膜超微病理组织的逆转效应  

Intervention of Yiqi Jiedu decoction on ultrastructural pathological tissue form nasopharynx with precancerous lesion

作　　者：吴新正[1] 戴娜[2] 田道法[3] 何迎春[4,5] 刘刚[1] 刘湘[4] 

机构地区：[1]湖南省第二人民医院耳鼻喉-头颈外科,湖南长沙410006 [2]湖南中医药大学第二中医临床学院,2008级湖南长沙410208 [3]湖南中医药大学第一附属医院头颈外科,湖南长沙410007 [4]湖南中医药大学中西结合学院,湖南长沙410007 [5]南华大学医学院,湖南衡阳421001

出　　处：《湖南中医药大学学报》2012年第3期6-9,共4页Journal of Hunan University of Chinese Medicine

基　　金：国家自然科学基金资助项目(81072904;30973856);中国博士后基金(2011M501277);湖南省科技计划资助项目(2010SK3021;2010TT2020);湖南省中医五官科学重点学科和湖南省2008年研究生创新基金研究项目

摘　　要：目的研究TgN(p53mt-LMP1)/HT转基因小鼠癌前病变鼻咽黏膜超微结构特征并观察益气解毒方的干预效应。方法将模型鼠随机分为3组,每组24只,模型组(MG)、益气解毒方组(YG)、维A酸组(TG);同品系C57BL/6J鼠24只作正常对照组(NG)。各组均单用诱癌剂二亚硝基哌嗪73 mg/(kg.次),每周2次,连续14周。采用HE染色法和透射电镜观察各组小鼠鼻咽黏膜形态学变化。结果 MG组鼻咽黏膜异型性增生明显,TG、NG、YG鼻咽/鼻腔黏膜表现轻度增生。MG组细胞膜呈伪足样改变,线粒体变形、出现空泡样结构,YG、TG及NG上述改变不明显。结论线粒体的改变可能与鼻咽黏膜癌前病变相关,而益气解毒方可有效逆转转基因小鼠鼻咽黏膜癌前病变。Objective To investigate ultrastructural changes of the nasopharynx and the intervening effect of Yiqi Jiedu formula decoction on the precancerous lesion among transgenic mice TgN（p53mt-LMP1）/HT.Methods 72 transgenic mice of TgN（p53mt-LMP1）/HT were randomly divided into 3 groups as follows： model group（MG）,Yiqi Jiedu group（YG） and retinoic acid group（TG）,24 mice in each group,and other 24 C57BL/6J mice were selected as the possitive control group（NG）.N,N＇-dinitrosopiperazine（DNP） was given to all the mice in a dose of 73mg/kg each time,two times per week and lasted for 14 weeks.The pathological changes of nasopharynx were observed with HE staining and transmission electron microscope.Results The nasopharyngeal epithelia hyperplasia was obvious in MG and some plasma membrane had the structure ofpseudopodium,and some mitochondria was deformation or was of bubble structure.YG、TG and NG only had slightly hyperplasia.Conclusion There is relation between the morphological changes of mitochondria and precancerous lesion in nasopharynx.The development of precancerous lesion in nasopharynx may be reversed by Yiqi Jiedu formula decoction.

关 键 词：鼻咽癌前病变 超微结构 益气解毒方 小鼠 干预 

分 类 号：R285.5[医药卫生—中药学]