云龙黑木耳中多糖的提取与测定  

Extraction and Determination of Polysaccharides from Yunlong Auricularia auricular

作　　者：倪兆林[1] 申元英[1,2] 白丽[2] 陈红云[1,2] 李润兵[1] 

机构地区：[1]大理学院公共卫生学院,云南大理671000 [2]大理学院基础医学院,云南大理671000

出　　处：《大理学院学报（综合版）》2012年第3期31-34,共4页Journal of Dali University

基　　金：大理学院大学生科研基金资助项目(2008DXS131)

摘　　要：目的：提取云龙野生和人工培养两种黑木耳多糖并测定其含量。方法：采用乙醇浸提法提取多糖,紫外分光光度苯酚-硫酸显色法测定粗多糖含量。结果：在8.0-48.0μg/mL线性范围内,回收率为99.03%,RSD为0.66%（n=6）,测到野生和人工培养黑木耳中多糖的收率分别为18.48%和18.29%;野生和人工培养黑木耳中多糖的含量分别为9.21%和8.20%。结论：野生黑木耳比人工培养黑木耳中多糖的含量高,生长环境对黑木耳多糖含量有影响。Objective： To extract both artificial culture and wild Auricularia auricular polysaccharides and determinate their contents.Methods： The polysaccharide was extracted with ethanol and the polysaccharide content was wired by UV spectrophotometry phenol-sulfuric acid color method.Results： Within the range of 8.0-48.0 μg/mL,the recovery ratio of polysaccharide was 99.03% with RDS of 0.66%（n=6）.The recovery ratio of polysaccharide in artificial culture and wild Auricularia auricular were 18.48% and 18.29%,respectively.Artificial culture and wild Auricularia auricular in the polysaccharide content was 9.21% and 8.20%,respectively.Conclusion： Wild Auricularia auricular has higher content of polysaccharides than that of culture artificially.Growing environment affects the contents of Auricularia auricula＇s polysaccharides.

关 键 词：黑木耳 乙醇浸提法 紫外分光光度苯酚-硫酸显色法 多糖 含量 

分 类 号：R284.2[医药卫生—中药学]