组胺对脑细胞内游离钙浓度的影响及其机制  

Effect of histamine on free intracellular calcium in isolated neonatal rat brain cells

作　　者：吕华瑞[1] 侯玉立[2] 汤允昭[1] 

机构地区：[1]山西医科大学药理学教研室,太原030001 [2]山西医科大学第一临床医学院神经内科

出　　处：《山西医科大学学报》2000年第2期107-108,共2页Journal of Shanxi Medical University

摘　　要：目的　观察组胺对脑细胞内游离钙浓度的影响 ,并初步探讨其作用机制。方法　利用荧光探针Fura - 2测定乳鼠脑细胞内游离钙的浓度。结果　组胺可明显升高脑细胞内游离钙浓度 ,而异丙嗪可以阻断组胺的这一效应。结论　组胺升高脑细胞内游离钙浓度是由H1受体介导的。Objective To study the effect of histamine on free intracellular calcium in isolated neonatal rat brain cells and its possible mechanism. Method The Ca 2+ -sensitive fluorescence indicator Fura-2 was used. Results Histamine(0 1～ 0 3 mmol/L ) improved [Ca 2+ ]i by 37 17%,66 99% and 94 29%,respectively.This effect of histamine on [Ca 2+ ]i was completely antagonized by promethazine( 0 1 mmol/L ). Conclusion These results suggest that histamine can elevate [Ca 2+ ]i and may be relate to the brain ischemic injure,whereas promethazine can antagonize the increase of [Ca 2+ ]i induced by histamine. [WT5”HZ]

关 键 词：组胺 脑细胞内 游离钙 异丙嗪 

分 类 号：R971[医药卫生—药品]