麦冬对脂多糖作用下大鼠腹膜间皮细胞TNF-α和TGF-β_1表达分泌的影响  

Effects of Ophiopogon on TGF-β_1 and TNF-α Expression and Secretion in Rats' Peritoneal Mesothelial Cell Stimulated by Lipopolysaccharide

作　　者：王秀梅[1] 姚菲菲[1] 孙立秋[1] 崔蕊[1] 王莉[1] 

机构地区：[1]哈尔滨医科大学附属第四医院,黑龙江哈尔滨150010

出　　处：《中医药学报》2012年第2期17-20,共4页Acta Chinese Medicine and Pharmacology

基　　金：黑龙江省自然科学基金(编号:D200852)

摘　　要：目的:考察麦冬对大鼠腹膜间皮细胞(RPMC)在脂多糖(LPS)作用下肿瘤坏死因子-α(TNF-α)和转移生长因子-β1(TGF-β1)表达分泌的影响。方法:原代培养鉴定RPMC;RT-PCR和ELISA法来分析麦冬对LPS影响下RPMC表达分泌TNF-α和TGF-β1的影响。结果:LPS能够明显增加RPMC表达分泌TNF-α和TGF-β1,而不同浓度的麦冬能降低LPS所致的RPMC的TNF-α和TGF-β1mRNA在转录和翻译水平的上调,这种抑制作用具有剂量依赖性。结论:麦冬能减弱LPS上调RPMC表达炎症因子TNF-α和TGF-β1的作用,进而促进RPMC的增殖修复,控制腹膜炎症,为中药改善腹膜间皮细胞损伤和防治腹膜透析相关腹膜炎及腹膜纤维化提供实验依据。Objective：To observe the effect of ophiopogon on cell tumor necrosis factor-α（TNF-α） and transforming growth factor-β1（TGF-β1） expression in rats＇ peritoneal mesothelial cell（RPMC） stimulated by lipopolysaccharide（LPS）.Method：RPMCs were isolated after enzymatic digestion,cultivated,and identified by phase contrast inverted microscope and immunocytochemistry method.Expressions of TGF-β1 and TNF-α were observed by RT-PCR,and concentration of TGF-β1 and TNF-α in cultivated medium was assayed by ELISA.Results： LPS induced TGF-β1 and TNF-α expression in RPMC.These changes could be partially inhibited by different concentrations of ophiopogon injection.This inhibition was dose-dependent.Conclusion： Ophiopogon reverses the higher expression of fibrosis cytokine TGF-β1 and TNF-α in RPMCs induced by LPS,through which peritoneal damage is repaired and the inflammation is improved.Therefore,our experimental evidences show the prevention and treatment approaches for peritoneal mesothelial cell injury and dialysis-related peritonitis.

关 键 词：脂多糖 麦冬 腹膜间皮细胞 中药 

分 类 号：R285.5[医药卫生—中药学]