玉米杂交种苏玉16农艺性状的QTL分析  

QTL mapping for agronomic traits of maize hybrid Suyu 16

作　　者：汤在祥[1,2] 贾波[1] 张丹[1] 陆鑫[1] 邓德祥[1] 徐辰武[1] 

机构地区：[1]扬州大学,江苏省作物遗传生理重点实验室,教育部植物功能基因组学重点实验室,江苏扬州225009 [2]苏州大学,苏州大学医学部公共卫生学院,江苏苏州215123

出　　处：《江苏农业学报》2012年第2期265-273,共9页Jiangsu Journal of Agricultural Sciences

基　　金：国家重大基础研究发展规划(973计划)项目(2011CB100106);国家自然科学基金项目(30971846、31100882);江苏省高校自然科学基金重点项目(09KJA210002)

摘　　要：采用强优势玉米杂交种苏玉16(JB×Y53)的两个亲本自交系,配置F2和相应F2∶3作图群体。利用154个SSR标记构建了分子标记连锁图谱,覆盖全基因组1 735.0 cM,标记间平均图距为11.3 cM。同时考察F2和F2∶3群体的株高、穗位高、抽穗期和散粉期等共10个重要农艺性状,采用联合F2和F2∶3群体的作图方法定位有关QTL。此外,采用QTL Cartographer V2.5软件分别对F2和F2∶3群体进行了有关QTL的重演性验证。结果表明,采用联合作图方法在调查的10个性状上共定位到93个QTL,采用QTL Cartographer V2.5软件共定位到96个QTL,其中56个能用两种方法重演验证。The maize inbred lines JB and Y53,the parental lines of maize hybrid Suyu 16,were used to create F2 and F2∶3 segregation populations.The genetic linkage map was constructed containing 154 SSR markers,which covered 1 735.0 cM of maize genome.The average distance of the flanking markers was 11.3 cM.The phenotypic value of 10 traits including plant height,ear height,heading date,pollen shedding,etc were investigated in F2 and F2∶3 populations.The QTL associated with these traits were mapped through jointing F2 and F2∶3 populations.The composite interval mapping（CIM） in software QTL Cartographer V2.5 was also used to map the QTL in F2 and F2∶3 populations,respectively.93 QTLs were identified in the 10 traits by joint mapping method,and 96 QTLs were identified by CIM method.56 QTLs among 93 QTLs could be verified by CIM method.

关 键 词：玉米 数量性状基因座位 农艺性状 联合作图 

分 类 号：S513.035.1[农业科学—作物学]