毛竹花粉活力测定及其适宜保存条件研究  

Pollen Vitality Test and Conservation Methods of Moso Bamboo

作　　者：王青[1,2] 彭祚登[1] 郭起荣[2] 冯云[2] 孙立方[2] 

机构地区：[1]北京林业大学,北京100083 [2]国际竹藤网络中心,北京100102

出　　处：《竹子研究汇刊》2012年第1期23-26,32,共5页Journal of Bamboo Research

基　　金：国家十二五科技支撑"竹藤资源培育与高附加值加工利用技术研究";国家自然科技资源条件平台"林木(含竹藤花卉)种质资源标准化整理;整合及共享试点"

摘　　要：试验立足于毛竹花粉种质的保存,得出毛竹花粉萌发的适宜条件为25℃、15%蔗糖+0.001%硼酸培养1 h,萌发率可达92.5%。适合毛竹花粉较长时间保存的条件为:新采集的花药,室内阴干1 h,离心管密封保存于4℃冷藏室,可维持80 h的活力。零下低温保存毛竹花粉技术需要进一步探索。Aimed at the germplasm conservation of Moso pollen,this study explored the condition suited for its germination and conservation.The results indicated that the suitable conditons for its germination were 15% sugar ＋ 0.001% boric acid at 25℃ for 1h.The germination rate could reach 92.5%.The suitable conditions of pollen conservation were that the new collecting anthers dried for 1h in room and then conserved in 4℃ freezer with sealed centrifuge tubes.That could maintain the vitality of pollen for 80h.The pollen conserved in subzero temperature and ultralow temperature（-196℃） lost their vitality.The technology of pollen preserved in subzero temperature needs further study.

关 键 词：毛竹 花粉活力 种质保存 萌发率 

分 类 号：S795.7[农业科学—林木遗传育种]