TaqMan聚合酶链反应技术检测结核分支杆菌DNA及其临床应用  

Detcetion of DNA extracted from Mycobacterium tuberculosis by TaqMan-PCR technique and its clinical application

作　　者：陈效友[1] 王洪平[2] 金玉生[2] 高薇薇[1] 张树才[1] 张伟[2] 马玙[1] 

机构地区：[1]北京市结核病胸部肿瘤研究所,101149 [2]中国医学科学院肿瘤研究所

出　　处：《中华结核和呼吸杂志》2000年第5期284-288,共5页Chinese Journal of Tuberculosis and Respiratory Diseases

摘　　要：目的　探讨TaqMan聚合酶链反应 (TaqMan PCR)技术在肺结核诊断中的价值。方法对 16 8例活动性肺结核、5 7例肺癌患者的痰和外周血及 34例健康对照外周血 ,同时应用TaqMan PCR、PCR检测 ,并与痰涂片法、BACTEC法及改良罗氏培养法结果进行比较。结果　TaqMan PCR检测痰和外周血总的阳性率分别为 5 3 0 %和 6 1 3% ,显著高于PCR、痰涂片法、BACTEC法及改良罗氏培养法 (P <0 0 5 )。TaqMan PCR检测痰和外周血特异性分别为 96 5 %和 94 7% ,与PCR检测痰和外周血的特异性为 93%相比 ,差异无显著性 (P >0 0 5 )。在 10 5例痰涂片阴性及痰培养阴性肺结核患者中TaqMan PCR检测外周血的阳性率达 45 7%。结论　TaqMan PCR具有较高的敏感性和特异性 ,对肺结核尤其痰涂片阴性及痰培养阴性肺结核的诊断有价值。Objective To evaluate the clinical diagnostic value of TaqMan PCR technique in the diagnosis of active pulmonary tuberculosis Methods The specimens of sputum and peripheral blood from 168 patients with active pulmonary tuberculosis, 57 patients with lung cancer and the specimens of peripheral blood from 34 healthy individuals were detected by TaqMan PCR, PCR The specimens of sputum were also detected by smear, culture with BACTEC and Lowenstein Jensen for comparison Results The positive rates of sputum and peripheral blood detected by TaqMan PCR were 53 0% and 61 3%, respectively, significantly higher than those of PCR, smear, culture with BACTEC and Lowenstein Jensen ( P <0 05) There was no significant difference between TaqMan PCR and PCR for specificity ( P >0 05) Of 105 specimens of peripheral blood from patients with smear negative and culture negative pulmonary tuberculosis, 48 specimens of peripheral blood detected by TaqMan PCR were positive The positive rate was 45 7% Conclusions TaqMan PCR method showed higher sensitivity and specificity It is an useful tool for diagnosis of pulmonary tuberculosis, particularly smear negative and culture negative pulmonary tuberculosis

关 键 词：结核 分支杆菌 聚合酶链反应 DNA 

分 类 号：R446.5[医药卫生—诊断学]