大孔树脂分离纯化沙芥黄酮的工艺研究  

Purification of flavonoids extracted from Pugionium cornutum(L.) Gaerth.by macroporous resins

作　　者：温俊峰[1] 刘步明[1] 

机构地区：[1]榆林学院化学与化工学院,陕西榆林719000

出　　处：《应用化工》2012年第5期827-830,共4页Applied Chemical Industry

基　　金：榆林市科技计划项目(Ng09-1-05);榆林学院院级科研项目(09-YK30)

摘　　要：用大孔吸附树脂分离纯化沙芥总黄酮,比较了7种大孔树脂对沙芥总黄酮的静态吸附动力学特性,优选出D-4020型大孔吸附树脂分离纯化沙芥总黄酮,并对其进行动态吸附实验。结果表明,D-4020纯化沙芥总黄酮的最佳工艺参数为:上样液浓度0.4 mg/mL,pH值5,上样流速2 mL/min;使用4BV用量95%的乙醇作为洗脱剂,洗脱流速为2 mL/min。采用该工艺分离纯化沙芥总黄酮含量达40.91%。A method of separation and purification of flavonoids from Pugionium cornutum（L.） Gaerth.was studied.The suitable resin D-4020 was choosed by static dynamic adsorption experiments of seven macroporous resin.The adsorption experiment of D-4020 resin was done to determine the best condition.The optimal purification conditions were as follows：the sample solution concentration 0.4 mg/mL,pH 5,the sample flow rate at 2 mL/min;the elution solvent 4BV of 95 % ethanol,the flow rate at 2 mL/min.Under above condition,the purity of flavonoids could reach to 40.91%.

关 键 词：沙芥 总黄酮 分离纯化 大孔树脂 

分 类 号：TQ461[化学工程—制药化工] TS201[轻工技术与工程—食品科学]