人外周血淋巴细胞Fas的表达及细胞凋亡的诱导  

Apoptosis of peripheral blood lymphocytes activated by allospecific antigen

作　　者：王良利[1] 邹萍[1] 胡中波[1] 

机构地区：[1]同济医科大学附属协和医院血液科,武汉430022

出　　处：《中华器官移植杂志》2000年第3期156-157,共2页Chinese Journal of Organ Transplantation

基　　金：国家自然科学基金资助项目! ( 39770 767)

摘　　要：目的　研究Fas系统对外周血淋巴细胞的影响。方法　将分离、纯化后的淋巴细胞进行单向混合淋巴细胞培养 ,用流式细胞术动态检测新鲜分离和激活后的T淋巴细胞表面Fas表达水平 ,并用DNA电泳和原位末端标记技术分别从定性和定量的角度观察Fas系统激活与否对混合培养后淋巴细胞凋亡率的影响。结果　T淋巴细胞在被激活后Fas表达水平升高 ,加抗Fas单克隆抗体组的细胞凋亡率高于未加抗体的对照组 (P <0 .0 5 )。Objective In order to study the effect of the Fas system on normal peripheral blood lymphocytes (PBL) activated by allospecific antigen. Methods The isolated and purified lymphocytes were subjected to one way mixed lymphocyte culture. Fas antigen expression on the surface of T lymphocytes freshly isolated and activated was detected dynamically by using flow cytometry (FCM). The influence of the Fas system activated or not on the rate of lymphocytic apoptosis after mixed culture was investigated by using DNA electrophoresis and TdT mediated dUTP nick end labeling (TUNEL). Results The Fas expression on the surface of activated T lymphocytes was increased and the rate of apoptosis of the group with addition of anti Fas monoclonal antibody was higher than that of the group without addition of antibody ( P < 0.05 ). Conclusion It was possible to dispose of the lymphocytes activated by specific antigen in vitro by anti Fas monoclonal antibody.

关 键 词：淋巴细胞 抗原 基因表达 FAS 细胞凋亡 

分 类 号：R392.4[医药卫生—免疫学]