宁波地区大肠埃希菌和肺炎克雷伯菌质粒AmpC酶基因检测  

Detection of plasmid-mediated AmpC β-lactamases genes in Escherichia coli and Klebsiella pneumoniae in Ningbo area

作　　者：顾欣 毛雄英[2] 王卫华[2] 汪丽[2] 吕婉飞[2] 

机构地区：[1]宁波市中心血站检验科,浙江宁波315040 [2]宁波大学医学院附属医院,浙江宁波315020

出　　处：《现代医药卫生》2012年第9期1298-1299,共2页Journal of Modern Medicine & Health

摘　　要：目的了解近年宁波地区大肠埃希菌和肺炎克雷伯菌临床分离株中质粒AmpC酶基因流行状态。方法用K-B法对临床分离的大肠埃希菌和肺炎克雷伯菌作耐药表型分析,通过聚合酶链反应(PCR)检测AmpC酶基因,然后用三维试验予以证实。结果 140株大肠埃希菌和肺炎克雷伯菌中,质粒AmpC酶基因阳性率2009、2010、2011年分别为4.4%(2/45)、8.6%(3/35)、13.3%(8/60);其中2009和2010年所检出的均为DHA基因型,2011年分别从2株大肠埃希菌和1株肺炎克雷伯菌中检出ACT-1基因。结论宁波地区临床分离的大肠埃希菌和肺炎克雷伯菌中质粒AmpC酶基因的流行率有增长趋势。Objective To investigate the prevalence and genotype of plasmid-mediated AmpC β-1actamases genes in Escherichia coli and Klebsiella pneumoniae strains isolated from Ningbo area. Methods After the detection for drug resistant phenotypcs by K-B method ,positive strains were subjected by polymerase chain reaction （PCR） ,then qualified through three-dimension test. Results From 2009 to 2011 ,the positive rates of plasmid-mediated AmpC genes were 4.4% （2/45）,8.6% （3/35）, 13.3% （8/60）,respectively.Meanwhile, the unique genotype was DHA for all positive strains in 2009 and 2010.In 2011, ACT-1 genotype was detected in 2 strains of Escherichia coli and 1 strain of Klebsiella pneumoniae. Conclusion The plasmid mediated AmpC genes in strains of Escherichia coli and Klebsiella pneumoniae clinically isolated in Ningbo area Eseherichia coli and Klebsiella pneumoniae show an increasing tendency in recent years.

关 键 词：埃希菌属 克雷伯菌 肺炎 质粒 头孢菌素酶 基因 

分 类 号：R378.2[医药卫生—病原生物学]