基于纳米粒子的比色检测及其在化学传感及生物检测的应用  

Nanoparticle-based Colorimetric Sensing Assay and Its Applications in Chemical and Biological Detections

作　　者：周化岚[1] 翟莲娜[1] 缪煜清[1,2] 黎莹[1] 施文健[1] 

机构地区：[1]上海理工大学环境与建筑学院,上海200093 [2]浙江师范大学化学与生命科学学院,浙江金华321004

出　　处：《分析测试学报》2012年第5期622-627,共6页Journal of Instrumental Analysis

基　　金：上海市教委科研创新项目(09YZ220)

摘　　要：基于纳米粒子的比色检测由于其简单性、多样性而备受关注。在该体系中,目标分析物直接或间接诱发纳米粒子的聚集和分散,从而可通过溶胶的颜色改变实现分析检测。该综述介绍了纳米粒子分散及聚集过程的物理现象,简单讨论了纳米粒子间的作用力,对两种作用机制(粒子间交联和非粒子间交联来调节纳米粒子的分散和聚集)进行了介绍。并对纳米粒子在重金属离子检测、生物检测等方面的应用及方法的分析特性进行了概述,对其未来发展趋势进行了展望。Nanoparticle-based colorimetric sensing assays have recently attracted considerable attention in diagnostic application due to their simplicity and versatility.The key to establish nanoparticle-based colorimetric sensing assay is the control of nanoparticle dispersion and aggregation by using analytes of interest.Consequently,the color of the solution is the central in the design of such systems.This paper introduced briefly the physical phenomenon（i.e.colors） associated with nanoparticles and their aggregation,discussed the interparticle forces of nanoparticles,and described the modulation of nanoparticle stability and aggregation,which utilized both interparticle crosslinking and noncrosslinking aggregation mechanisms.The applications of nanoparticle-based colorimetric sensing assay in detecting heavy metal ions and biomolecules were reviewed,and its prospect was put forward.

关 键 词：纳米粒子 比色 重金属离子 生物分子 化学传感 生物检测 综述 

分 类 号：O657.3[理学—分析化学] G353.11[理学—化学]