双歧杆菌丝状体生长形态初探  

Initial Study on Growth Morphology of Bifidobacterium Filaments

作　　者：刘艳霞[1] 刘蕾[1] 刘俊康[1] 邓渝[1] 

机构地区：[1]第三军医大学医学检验系生物波研究中心,重庆400038

出　　处：《微生物学杂志》2012年第2期94-97,共4页Journal of Microbiology

摘　　要：研究双歧杆菌在特定培养条件下生长及增殖模式的变化。采用不同浓度维生素B6和维生素E烟酸酯药敏纸片联合影响双歧杆菌生长,通过革兰染色和原子力显微镜扫描观察双歧杆菌的生长、增殖情况。在维生素B6和维生素E联合作用下,双岐杆菌出现丝状体群集生长的现象,且二分裂增殖方式受到抑制。第1次转种于普通培养基中可保持此种生长增殖方式,但连续转种3次后会恢复常规生长增殖方式。不同浓度维生素B6和维生素E烟酸酯药敏纸片联合可诱生双歧杆菌丝状体。The changes of the growth and proliferation patterns of bifidobacterium under specific culture conditions were studied.Different concentrations of VB6 and VE nicotinate of drug-sensitive paper were adopted to jointly affect the growth of bifidobacterium.The growth and proliferation of bifidobacterium were observed through scanning atomic force microscope after Gram staining.The results showed that under joint affection of VB6 and VE,a phenomenon of bifidobacterium filaments cluster emerged,and the bifid proliferation pattern was inhibited.The cluster growth and proliferation of bifidobacterium could maintain in the first transferred culture,but after three consecutive transferred cultures bifidobacterium resumed to normal proliferation pattern.Therefore,different concentrations of VB6 and VE nicotinate drug sensitive paper could jointly induce bifidobacterium filaments.

关 键 词：双歧杆菌 丝状体 群集生长 二分裂增殖 

分 类 号：Q934[生物学—微生物学]