小儿清肺化痰口服液中3种有效成分的CE法测定  

Determination of three effective constituents in Xiao'er Qingfei Huatan Koufuye by CE

作　　者：唐冰雯[1] 李雯婷[1] 宋粉云[1] 

机构地区：[1]广东药学院药科学院,广东广州510006

出　　处：《广东药学院学报》2012年第2期156-159,共4页Academic Journal of Guangdong College of Pharmacy

基　　金：广东省自然科学基金资助项目(5002841)

摘　　要：目的采用毛细管电泳法(CE法)测定小儿清肺化痰口服液中盐酸麻黄碱、盐酸伪麻黄碱和盐酸甲基麻黄碱的质量分数。方法采用未涂层弹性融硅石英毛细管柱(67 cm×75μm,有效长度60 cm);以30 mmol/L Na2B4O7-甲醇(体积比9∶1)为运行缓冲液;分离电压为10 kV;检测波长190 nm;重力进样10 s(高度15 cm);以盐酸巴马汀为内标。结果盐酸麻黄碱、盐酸伪麻黄碱和盐酸甲基麻黄碱质量浓度分别在2.4～30.0μg/mL、1.2～15.0μg/mL、0.4～5.0μg/mL范围内线性关系良好,平均回收率分别为93.8%、96.3%、95.8%。结论该方法简单、准确、重复性较好,可用于小儿清肺化痰口服液的质量评价和控制。Objective To establish a capillary electrophoresis method for the separation and analysis of ephedrine hydrochloride,pseudoephedrine hydrochloride and methyl ephedrine hydrochloride in Xiao＇er Qingfei Huatan Koufuye.Methods An uncoated fused sillica capillary column（67 cm×75 μm,effective length 60 cm）was used with running buffer of 30 mmol/L NaB4O7-CH3OH（9：1）,at the applied voltage of 10 kV and detection wavelength of 190 nm.Samples were injected by gravity of 10 s（15 cm） and palmatin hydrochloride was used as the internal standard.Results Ephedrine hydrochloride,pseudoephedrine hydrochloride and methyl ephedrine hydrochloride showed good linearity in the concentration range of 2.4-30.0 μg/mL,1.2-15.0 μg/mL,0.4-5.0 μg/mL,respectively.The average recovery ratio of three active ingredients were 93.8%,96.3%,95.8%.Conclusion The method is simple,accurate and reproducible,which can be used for quality control of Xiao＇er Qingfei Huatan Koufuye.

关 键 词：毛细管电泳法(CE法) 小儿清肺化痰口服液 盐酸麻黄碱 盐酸伪麻黄碱 盐酸甲基麻黄碱 

分 类 号：R286.0[医药卫生—中药学]