丹参酮ⅡA对前列腺成纤维细胞增殖及胶原表达的影响  

Effects of Tanshinone ⅡA on proliferation of prostate fibroblasts and expression of collagen genes

作　　者：刘昆云[1] 刘顺会[1] 徐晓鹏[2] 余琳俐[1] 陈金祥[1] 

机构地区：[1]广东药学院生命科学与生物制药学院,广东广州510006 [2]中山大学生命科学学院,广东广州510275

出　　处：《广东药学院学报》2012年第2期200-203,共4页Academic Journal of Guangdong College of Pharmacy

基　　金：国家自然科学青年基金(30901114)

摘　　要：目的研究丹参酮ⅡA对转化生长因子-β1(TGF-β1)诱导的前列腺成纤维细胞增殖及Ⅰ、Ⅲ型胶原表达的影响,初步探讨丹参酮ⅡA是否具有治疗前列腺组织纤维化的潜力。方法通过消化法原代培养和差速贴壁法纯化获得PrF,分别用5 ng/mL的TGF-β1和不同质量浓度的丹参酮ⅡA(25、2.5、0.25mg/L)对其进行处理,噻唑蓝(MTT)比色法检测细胞增殖活性,半定量反转录-聚合酶链式反应(RT-PCR)检测Ⅰ、Ⅲ型胶原基因的表达。结果 5 ng/mL TGF-β1可明显诱导PrF的增殖及Ⅰ、Ⅲ型胶原基因的表达(P<0.01)。25、2.5、0.25 mg/L质量浓度的丹参酮ⅡA均能显著抑制TGFβ1诱导的细胞异常增殖(P<0.05或P<0.01),且呈良好的量效关系。25 mg/L的丹参酮ⅡA对Ⅰ、Ⅲ型胶原基因的表达有抑制作用(P<0.01)。结论丹参酮ⅡA可抑制TGF-β1诱导的PrF异常增殖和Ⅰ、Ⅲ型胶原基因的表达,具有抗前列腺纤维化的潜力。Objective To study the effects of tanshinoneⅡA on the model established by TGF-β1-induced prostate fibroblasts（PrF） proliferation and expressions of type Ⅰ,Ⅲ collagen genes,which can help reveal whether tanshinone ⅡA have the potential anti-prostate fibrosis function.Methods Neonatal rat PrF were harvested by trypsin digestion and differential attachment and treated with 5 ng/mL TGF-β1 and different concentrations of tanshinoneⅡA（25,2.5 and 0.25 mg/L）.PrF proliferation was detected by thiazolyl blue（MTT）assay,and the expression of type Ⅰ,Ⅲ collagen genes was determined by reverse transcription-polymerase chain reaction（RT-PCR）.Results PrF proliferation and expression of type Ⅰ,Ⅲ collagen genes increased significantly after TGF-β1 treatment（P0.01）.TanshinoneⅡA（25,2.5 and 0.25 mg/L） downregulated PrF proliferation（P0.05 or P0.01） induced by TGF-β1 in a dose-dependent manner.Peak concentration of TanshinoneⅡA（25mg/L） can remarkably inhibited the expressions of type Ⅰand Ⅲ collagen genes by RT-PCR（P0.01）.Conclusion These findings demonstrate that TanshinoneⅡA against prostate fibrosis was likely related to its inhibition of TGF-β1-induced PrF proliferation and expressions of type Ⅰ,Ⅲ collagen genes.

关 键 词：丹参酮ⅡA 转化生长因子-β1(TGF-β1) 前列腺组织纤维化 前列腺成纤维细胞(PrF) 

分 类 号：R285[医药卫生—中药学]