浙江产玄参不同采收季节哈巴俄苷和肉桂酸含量变化分析  

Contents Variety of Harpagoside and Cinnamic Acid in Radix Scrophulariae Collected from Zhejiang Province in Different Seasons

作　　者：刘洪宇[1] 钮正睿[2] 蔡铁全[1] 

机构地区：[1]国家食品药品监督管理局保健食品审评中心,北京100070 [2]中国食品药品检定研究院食品化妆品检定所

出　　处：《中国药师》2012年第5期613-616,共4页China Pharmacist

摘　　要：目的:测定浙江产栽培玄参不同采收期、不同植物部位以及不同采收期野生玄参主根中哈巴俄苷和肉桂酸的含量。方法:Zorbax ODS分析柱(250 mm×4.6 mm,5μm),流动相为乙腈和1%醋酸水溶液线性梯度洗脱(20:80:50:50)(20min),流速为1 ml·min^(-1),室温,检测波长为278 nm。结果:哈巴俄苷与肉桂酸回收率分别为97.7%和102.3%,RSD分别为0.68%和1.93%。测定了浙江产16份玄参样品中哈巴俄苷和肉桂酸的含量。结论:野生玄参和栽培玄参的次生代谢产物累积的周期不同,11月份采收的野生玄参中哈巴俄苷的含量比较高;而9月份采收的栽培玄参中哈巴俄苷的含量比较高。根茎、子芽也可以考虑作为玄参的药用部位。Objective：To determine the content of harpagoside and cinnamic acid in wild and planted Scrophularia ningpoensis collected from Zhejiang province at different collecting times.Method：An HPLC method was established.The experimental conditions were as follows;a Zonbax ODS column（250 mm×4.6 mm,5μm）,the mobile phase of acetonitrile-water（containing 1.0%acetic acid）（20：80→50：50）（20 min）,the flow-rale of 1 ml ？ min^（-1） with room temperature and the detection wavelength of 278 nm.Result： The recovery and RSD were 97.7%and 0.68%for harpagoside,and 102.3%and 1.93%for cinnamic acid.16 samples of Scrophularia ningpoensis from Zhejiang province were quantitatively analyzed and the contents of harpagoside and cinnamic acid were determined.Conclusion：Scrophularia ningpoensis shows the different secondary metabolism accumulation.The wild Scrophularia ningpoensis collected in November has higher content of harpagoside than the other wild ones.The planted Scrophularia ningpoensis collected in Sepember exhibits higher content of harpagoside than the other planted ones.The rhizome and bud can be used as the resources of harpagoside as well.

关 键 词：玄参 哈巴俄苷 肉桂酸 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]